Researchers develop increasingly complex mini-brains

March 8, 2019, D'Or Institute for Research and Education
75 days old brain organoid with staining for neurons (green) and astrocytes (red). Credit: D'Or Institute for Research and Education.

Scientists of the D'Or Institute for Research and Education have improved the initial steps of a standard protocol and produced organoids displaying regionalized brain structures, including retinal pigmented cells. Their results are published in BMC Developmental Biology.

Human brain organoids are aggregates formed by nervous cells obtained from cell reprogramming. Via this technique, cells extracted from skin or urine of volunteers are transformed into and then into neurons and other nervous cell types. They are cultivated for weeks, until they start forming agglomerates that resemble an embryonic brain.

For the past few years, scientists have been trying to perfect this model in order to create increasingly complex organoids similar to those at later stages of development. Since 2016, in with the Federal University of Rio de Janeiro (UFRJ), scientists from the D'Or Institute have cultivated human brain organoids to study and the effects of new drugs on the nervous system.

They put in a nutrient-rich liquid, similar to the development environment of the human embryo. From there, the mini-brains develop in a self-regulated process. In other words, all researchers must do is make sure they have the right environment to develop.

Recently, the team lead by Stevens Rehen has been able to refine the environment in which the cells are maintained. "These organoids are a demonstration that it is possible to repeat in the laboratory increasingly advanced gradients of human brain development," says Rehen. "We developed a cost-effective suspension method on orbital steering plates as an alternative for the cultivation of brain organoids with retinal pigmented ."

Explore further: Biologists use 'mini retinas' to better understand connection between eye and brain

More information: Livia Goto-Silva et al, Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms, BMC Developmental Biology (2019). DOI: 10.1186/s12861-019-0183-y

Related Stories

Modular approach found to improve consistency of organoids

August 15, 2017

(Medical Xpress)—A team of researchers from the U.S. and Australia working at the Yale Stem Cell Center report that they have met with some success in improving the usefulness of organoids. In their paper published in the ...

Recommended for you

Coffee-based colloids for direct solar absorption

March 22, 2019

Solar energy is one of the most promising resources to help reduce fossil fuel consumption and mitigate greenhouse gas emissions to power a sustainable future. Devices presently in use to convert solar energy into thermal ...

EPA adviser is promoting harmful ideas, scientists say

March 22, 2019

The Trump administration's reliance on industry-funded environmental specialists is again coming under fire, this time by researchers who say that Louis Anthony "Tony" Cox Jr., who leads a key Environmental Protection Agency ...


Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.