Related topics: cell division

Defining the centromere

The division of cells is a highly-regulated and complex process which requires the organised collaboration of a multitude of different cellular components. Although the basic principles are known, many components and their ...

Scientists model a crucial component of cell division

During the process of cell division, chromosomes must be distributed equally between the two emerging daughter cells. One copy of each chromosome is created and remains glued to the original until threads, called microtubules, ...

Kinetochore structure reveals how it takes hold

( -- With the first-ever three-dimensional image of an isolated kinetochore – the bulky molecular machine that connects a chromosome to the long, thin microtubules that tug it to one end of a dividing cell -- ...

Chromosome centromeres are inherited epigenetically

Centromeres are specialised regions of the genome, which can be identified under the microscope as the primary constriction in X-shaped chromosomes. The cell skeleton, which distributes the chromosomes to the two daughter ...

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The kinetochore ( /kɪˈnɛtəkɔər/) is the protein structure on chromatids where the spindle fibers attach during cell division to pull sister chromatids apart.

The kinetochore forms in eukaryotes, assembles on the centromere and links the chromosome to microtubule polymers from the mitotic spindle during mitosis and meiosis.

"Monocentric" organisms, including vertebrates, fungi, and most plants, have a single centromeric region on each chromosome which assembles one kinetochore. "Holocentric" organisms, such as nematodes and some plants, assemble a kinetochore along the entire length of a chromosome.

The kinetochore contains two regions:

Kinetochores start, control and supervise the striking movements of chromosomes during cell division. During mitosis, which occurs after chromosomes are duplicated during S phase, two sister chromatids are held together each with its own kinetochore which face in opposing directions and attach to opposite poles of the mitotic spindle. Following the transition from metaphase to anaphase, the sister chromatids separate from each other, and the individual kinetochores on each chromatid drive their movement to the spindle poles that will define the two new daughter cells. Thus, the kinetochore is essential for the chromosome segregation that is classically associated with mitosis and meiosis.

Even the simplest kinetochores consist of more than 45 different proteins. Many of these proteins are conserved throughout eukaryote species, including a specialized histone H3 variant (called CENP-A or CenH3) which helps the kinetochore associate with DNA. Other proteins in the kinetochore attach it to the microtubules (MTs) of the mitotic spindle. There are also motor proteins, including both dynein and kinesin, which generate forces that move chromosomes during mitosis. Other proteins, such as MAD2 monitor the microtubule attachment as well as the tension between sister kinetochores and activate the spindle checkpoint to arrest the cell cycle when either of these is absent.

In summary, kinetochore functions include anchoring of chromosomes to MTs in the spindle, verification of anchoring, activation of the spindle checkpoint and participation in force generation to propel chromosome movement during cell division.

On the other hand, MTs are metastable polymers made of α- and β-tubulin, alternating between growing and shrinking phases, a phenomenon known as "dynamic instability". MTs are highly dynamic structures, whose behavior is integrated with kinetochore function to control chromosome movement and segregation.

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