Are viruses the new frontier for astrobiology?

They are the most abundant form of life on Earth, but viruses – or their seed-like dormant state, known as virions – are outliers in our search for life on other planets. Now, one group of scientists are pushing for astrobiologists to consider searching for viruses beyond Earth more seriously.

In NASA's current astrobiology strategy, viruses are mentioned six times in its 250 pages, write the authors of a recent paper Astrovirology: Viruses at large in the Universe. They call for the study of viruses to be incorporated into extraterrestrial science missions and astrobiological research at home, and have a checklist for the actions needed to put viruses on the interplanetary map.

"Viruses are an integral part of life on Earth as we know it," saysKen Stedman, a virologist at Portland State University's Center for Life in Extreme Environments and a co-author on the paper. If we are going to be thinking about life on early Earth or ancient or current life on other planets, we need to be thinking about viruses, he says.

It has been more than a century since scientists discovered the first virus, and for decades it was known simply as a "very small disease-causing agent". Late Nobel laureate Sir Peter Medawar even referred to them as "a piece of bad news wrapped up in a protein", Stedman and colleagues write.

Their current definition is more complicated, and less defamatory: viruses are entities whose genome replicates inside living cells, and are able to transfer that viral genome to other cells. As this definition implies, viruses comprise the whole reproduction cycle – and they need other living cells to reproduce. Virions, on the other hand, are the viral seedsthat could become viruses if they happen upon compatible living cells in which to replicate. On Earth, virions and viruses go hand-in-hand with life, and if we find the former on other planets they could point to cellular life once having existed on them.

On Earth, viruses are thought to outnumber cellular life forms by a factor of 10. And our planet is teeming with virions. In fact, a teaspoon of sea water can contain up to 50 million virions.

"It makes sense to be looking for the things that are likely to be the most abundant," says Stedman, who also chairs the Virus Focus Groupwithin NASA's Astrobiology Institute. "If an alien intelligence came to Earth looking for life, they would probably get a sample of sea water, loaded with virions. The alien life would come to the conclusion that Earth is inhabited by virions."

However, there are currently no extraterrestrial missions planning to hunt for virions in the water plumes on Jupiter's moon Europaor the jets of Enceladus.

"Astrovirology is no more, nor less, valid than astrobiology," says Don Cowan, director of the Centre for Microbial Ecology and Genomics at the University of Pretoria. "There is no reason why astrovirology should not be considered with the same emphasis as 'prokaryote' [i.e. bacterial] astrobiology, particularly since the lesson from Earth's biology is that every known organism has one or [sometimes many] more virus parasites."

Part of the reason for astrovirology's absence from space science agendas, Stedman says, is that virologists have not been reaching out to astrobiologists and pushing the case for virion hunting. Another major reason is technical: virions are tiny (with diameters ranging from 20 nanometers to over one micrometer), and so scientists need transmission electron microscopes to see their unique and varied shapes.

"While this may not be difficult on Earth, it seems unlikely that a transmission electron microscope will be put on a spacecraft in the foreseeable future," the authors note.

That was one of the authors' aims in writing their paper – to stimulate the development and testing of technologies that scientists could use in remote locations to discover virions.

"We need to look for biosignatures, and the morphologies of these virions are extremely distinctive," says Stedman.

However, another challenge is finding a way to look for unknown virions. "We have to be careful when trying to find genomes of things that we don't know are there," Stedman says. While there are techniques to identify known viruses, such as high throughput sequencing or high density microarrays, identifying truly novel virions could pose a problem, the authors write.

Scientists can hunt for extraterrestrial cellular life forms, such as bacteria or more complex life, by looking for certain chemicals on a planet or in its atmosphere, but virions do not create by-products like methane or oxygen. But, the authors hypothesize, if viruses had once infected life on other planets, perhaps those viruses changed the host organism's metabolism and this could be remotely detected from Earth. One possibility is that host organisms' chemical by-products may be slightly different to those that had not been infected by viruses. If this was the case, this could be a proxy for detecting cellular life and viruses.

In the short term, the astrovirologists suggest that, among other things, researchers need to find distinctive virus biosignatures; consider virus-detection experiments for Europa and Enceladus; and include virus models in our models for ancient oceans and other planets.

However, Stedman says that even if there were no virions on other planets, it would be an important discovery. "If we find somewhere else where living cells don't have viruses associated with them, [then] that would be something very different from Earth and very interesting."