April 29, 2019 feature
Engineering ECM-like fibers with bioactive silk for 3-D cell culture
Biological tissues are built when cells anchor to specific sites on a 3-D microfiber network in an extracellular matrix (ECM). Scientists are keen to recreate biological tissues in the lab using bioinspired tissue engineering and genetic engineering, to form functional ECM motifs fused to recombinant silk proteins. Under adequate physiological conditions, bioengineered silk proteins and fibronectin-silk (FN-silk) can self-assemble into microfiber networks that mimic native ECM.
In a recent study, Ulrika Johansson, Mona Widhe and co-workers at the interdisciplinary departments of Biotechnology, Biomaterials Chemistry, and Immunology in Sweden developed a method to include mammalian cells into a silk solution before assembling silk into constructs, to form uniform cell-integrated tissue-like microfibers. The resulting 3-D scaffold constructs showed improved cell proliferation (growth) and homogenous cell spreading compared to cells encapsulated in hydrogel. The results of the study are now published in Scientific Reports.
The scientists confirmed cell attachment on fibronectin-silk constructs (FN-Silk) in the work by observing filamentous actin and by defining focal adhesion points of the attached, elongated cells. They maintained cell viability for 90 days in the cell-FN/silk surfaces and showed scalability of the method to macro-sized 3-D cell cultures. The silk microfiber bundles with encapsulated cells maintained biomechanical strength and extendibility much like human arterial walls.
The protocol developed by Johansson and Widhe et al. also allowed stem cells to differentiate inside the 3-D constructs to assist the growth of diverse cell co-cultures. They showed that endothelial cells could be included into the bioinspired materials to form vessel-like structures throughout the tissue constructs. The scientists envision using the ECM-like network as a foundation for future efforts to engineer functional biological tissues in the lab.
In vitro mammalian cell culture is an indispensable experimental technique in basic research and industrial applications, although the existing process relies on 2-D hard plastic or glass surfaces for convenience—impairing the native biological response. Since biological cells are naturally accustomed to receiving signals from the 3-D environment, tissue engineers have formed new experimental strategies using 3-D cell cultures. The experimental conditions maintained cell adhesion, proliferation and differentiation to recreate and sustain cell metabolism and functionality in the lab.
Previously, Johansson and Widhe et al. had developed a scalable process to engineer the recombinant spider silk protein known as 4RepCT for bioinspired cell culture in the lab, which self-assembled into biodegradable and biocompatible microfibers in aqueous, physiological buffers at room temperature. They functionalized the novel construct using a cell adhesion motif from fibronectin (FN) to form the FN-silk material and promote firm cell attachment. Although cells proliferated along the new material surfaces, they remained on the surface alone, unable to proliferate in to the constructs to adequately mimic tissue-like properties in vitro. In the present work, the scientists therefore developed a new method, to efficiently embed cells in to the silk material during the assembly of FN-silk for encapsulated and viable 3-D cell culture that adequately mimicked the extracellular matrix in vitro.
Tissue engineering cell-embedded silk constructs in the lab
During the experiments, Johansson and Widhe et al., first added a drop of dispersed stem cells (mesenchymal mouse stem cells, MMSC) to the FN-silk protein solution prior to solution assembly. After incubation, the newly formed network remained stable in culture media and the encapsulated cell number increased in the constructs throughout the period of culture. After three days, the cells spread across all dimensions of the foam, which the scientists observed using differential interference contrast (DIC) microscopy.
They directed the cell alignment to form a random 3-D network of microfibers that successfully mimicked biological tissues by forming a macroscopic bundle of microfibers during the timeline of cell culture, followed by cell to ECM ratio examination. The scientists varied the ECM to cell composition to mimic or match a range of tissue types, including cell sparse and high-density tissues of the liver. The method is therefore also suited for 3-D cell culture with minute cell quantities.
Testing cell viability on the silk scaffolds – cytocompatibility studies
The scientists used growth profiles to map the diverse cell types embedded in both foam and fibers of the silk assembly. They observed an increased signal from the metabolic activity to represent cell proliferation in the 3-D silk scaffolds and in time they showed increased cell density in the innermost cell-silk scaffold. Johansson et al. investigated cell proliferation using BrdU staining, where positive results proved deeper proliferation and cell spreading in the silk fibers to maintain cell viability after 2 weeks of encapsulation, and during long-term cell culture periods that spanned one to three months.
Comparing the cytocompatibility of silk vs. hydrogel biomaterials
Based on the encouraging preliminary results, the scientists conducted parallel experiments to compare cell growth in silk vs. cell growth in hydrogel to determine cytocompatibility of the two materials. They chose alginate to represent the hydrogel during cell culture and observed dissimilarities between alginate vs silk, recording clear cell expansion in silk, while cells in alginate remained in a steady metabolic state. Using confocal microscopy, they investigated reasons for the observed difference in cell growth at the level of the microenvironment. The results showed prompt cell attachment (seen with elongated cells) in the silk constructs, while the alginate constructs contracted during cell culture, which may have stressed the cells to detach.
Biomechanical studies – characterizing the new materials
They determined material surface stiffness to be a crucial component that affected cell fate. To verify this observation, Johansson et al. tested biomechanical behavior of the silk constructs to ensure they adequately mimicked native tissue. They conducted tensile testing in a physiological buffer to obtain the results, which proved that the mechanical properties of silk containing cells matched those of connective tissue such as arterial walls. Johansson et al. were able to demonstrate high extendibility of the microfibers to indicate force transition into and throughout the cells attached to the new biomaterial – confirming adequate cell attachment.
Biofunctionalization studies – investigating surface biocompatibility in vitro
After establishing biomechanical stability, the scientists tested if the stem cells that grew on the silk scaffolds could differentiate (mature) on the same material. For this, they used pluripotent and multipotent human embryonic stem cells in the cell culture assays, followed by quantifying gene expression of biological markers of cell growth; FOXA2 (metabolic genes), SOX17 (genes for embryonic development and cell fate determination), CER1 (bone morphogenetic protein antagonists) and NANOG (embryonic stem cell proliferation, pluripotency and renewal). In the results, both SOX17 and CER1 showed robust upregulation, while the biomarker of pluripotency (NANOG) decreased due to cell maturity as a result of the loss of pluripotency.
The scientists tested surface biofunctionalization for diverse cell types, including human skeletal muscle satellite cells (HSkMSC) and bone marrow-derived human mesenchymal stem cells (hMSC). After cell expansion on the constructs, the scientists could steer the fate of the hMSCs into either adipogenic or osteogenic cell lineages. Additionally, after two weeks of cell culture, Johansson et al. showed myogenic differentiation of the HSkMSCs to form prominent actin filaments, and express the muscle-specific marker desmin, to verify in vitro myotube maturation.
Engineering biological vessels in the lab
The scientists then combined endothelial cells in the 3-D vascular network to form connective tissue that mimicked inherent cellular organization of micro-vessels in the lab. They followed the same protocol using silk assembly with cell integration and added a fraction of endothelial cells to engineer the connective tissue. In two weeks, they observed the cells gather and form millimeter long branched sprouts and vessel-like structures with prominent rings of endothelial cells in the silk fibers. The scientists could increase the size of the constructs to-scale and determine the alignment and aggregation of diverse cell types.
In this way, Johansson and Widhe et al. demonstrated a new strategy and developed a protocol to fit in functional cells within 3-D networks that mimicked the fibrous architecture of the native extracellular matrix (ECM). To mediate the experiment, they used self-assembling recombinant silk proteins and showed that a variety of cells could be embedded in the 3-D constructs. The setup and protocol are simple and cost-effective, unlike 3-D printing the process is frugal and hands-on, without expensive machinery. The scientists aim to optimize and standardize this protocol to develop biocompatible, advanced silk materials in tissue engineering. The experimental work will have a wide range of applications in materials science as miniature in vitro models for drug development and as larger bioengineered tissue constructs in regenerative medicine.
Brendon M. Baker et al. Cell-mediated fibre recruitment drives extracellular matrix mechanosensing in engineered fibrillar microenvironments, Nature Materials (2015). DOI: 10.1038/nmat4444
Darren Rodenhizer et al. A three-dimensional engineered tumour for spatial snapshot analysis of cell metabolism and phenotype in hypoxic gradients, Nature Materials (2015). DOI: 10.1038/nmat4482
Young's moduli and shear moduli in cortical bone. Proceedings of the Royal Society Series B: Biological Sciences. doi.org/10.1098/rspb.1996.0044
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