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Restoring the function of a human cell surface protein in yeast cells

Restoring the function of a human cell surface protein in yeast cells
The proposed approach involves causing random mutations on human derived receptor H3R and introducing the altered genetic code into yeast cultures. This allows for the convenient screening of mutations that restore the receptor’s normal function. Credit: Mitsunori Shiroishi from Tokyo University of Science

G protein-coupled receptors (GPCRs) are the largest and most diverse group of cell surface proteins in humans. These receptors, which can be seen as "traffic directors," transmit signals from the outside to the inside of cells and are involved in many physiological processes.

Given their prominent roles in cellular communication, , immune responses, and sensory perception, many drugs have been developed to target GPCRs, for the treatment of conditions such as asthma, allergies, depression, hypertension, and . In fact, more than 300 GPCR-related drugs are currently in clinical trials, 36% of which target over 60 novel GPCR targets without an already-approved .

Moreover, drugs that target GPCRs account for as much as 27% of the global market share of therapeutic drugs, with aggregated sales close to US$890 billion between 2011 and 2015. Thus, any technique that could accelerate research on GPCRs is likely to trigger a large ripple effect, ultimately bringing more effective treatments to millions of people.

Today, approaches such as cryo-, optogenetics, and , biosensors and label-free technologies, and single-cell technologies are being explored for GPCR drug discovery and development.

Among them, the single-cell approach based on yeast is one of the most useful platforms to study GPCRs. Besides its widespread application in beer and bread making, the yeast species Saccharomyces cerevisiae has a long history of being used as a host to research human derived GPCRs.

Although some GPCRs can be engineered to enhance their stability and function to facilitate experiments, most GPCRs do not function well in yeast cells. This long-standing problem has greatly slowed progress in our understanding of GPCRs and the development of new drugs that target them.

Against this backdrop, a research team from Tokyo University of Science (TUS), Japan, recently came up with an innovative strategy to restore the activity of human derived GPCR human histamine 3 (H3R) in S. cerevisiae. Their study, published in Scientific Reports on September 26, 2023, was led by Associate Professor Mitsunori Shiroishi and co-authored by Ms. Ayami Watanabe and Ms. Ami Nakajima, all from TUS.

"H3R is mainly expressed in the nervous system. It is involved in cognitive function, and its inhibition is associated with the therapeutic outcomes of various conditions, such as ADHD, schizophrenia, Alzheimer's disease, and narcolepsy," explains Dr. Shiroishi. Through preliminary experiments, the team showed that H3R becomes non-functional when expressed in yeast.

To restore its function, the research team utilized a technique called error-prone polymerase chain reaction to introduce in the H3R gene.

After producing a random mutant library of H3R, they introduced modified DNA segments into and cultivated them in the presence of an H3R agonist—a compound that binds to H3R and sets off a measurable response. By screening through multiple cultures, the researchers obtained four mutants in which the normal activity of H3R was restored.

These mutants responded exclusively to a type of yeast strain that harbors certain G-chimera proteins. The mutations responsible for the restored activity were located near the amino acid sequence motifs important for GPCR activation.

This innovative approach to study GPCRs could have profound implications, particularly in the fields of medicine and cell biology.

"Our research could help elucidate the function of GPCRs and may even lead to the development of drugs with fewer side effects, as well as bolster drug discovery for diseases for which there is currently no treatment," said Dr. Shiroishi.

There are many therapeutic areas where GPCR-targeting drugs are being actively developed, including neurological disorders like Alzheimer's and schizophrenia, cardiovascular diseases such as hypertension and heart failure, various types of cancer, and metabolic disorders.

A deeper understanding of GPCR variations and how they impact individuals differently could also lead to new approaches to personalized medicine. Tailoring GPCR-targeted drugs to an individual's genetic makeup and their specific disease profile may greatly improve treatment outcomes. Furthermore, generic GPCR treatments reaching a vast number of people worldwide might also become a reality, which would reduce the burden on health care systems.

More information: Ayami Watanabe et al, Recovery of the histamine H3 receptor activity lost in yeast cells through error-prone PCR and in vivo selection, Scientific Reports (2023). DOI: 10.1038/s41598-023-43389-z

Journal information: Scientific Reports

Citation: Restoring the function of a human cell surface protein in yeast cells (2023, October 19) retrieved 1 March 2024 from
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