Heredity matters: Ancestral protease functions as protein import motor in chloroplasts

October 22, 2018, Osaka University
Figure 1. Discovery of a novel import motor ATPase complex at the inner chloroplast envelope membrane. Credit: Osaka University

Over 1 billion years ago, a relationship began between the ancestor of all living plants and a type of bacterium that paved the way for the evolution of life as we know it. The single-celled algal ancestor engulfed, but crucially, did not destroy, a cyanobacterium-like organism with which it established a mutually beneficial bond. This symbiotic relationship provided energy in the form of sugars derived from photosynthesis (whereby sunlight is converted into chemical energy) from the cyanobacterium to its host.

The symbiont cell eventually became the first . Even today, these organelles hold on to structures from their early days as independent organisms, including their own DNA and a double . During evolution, several chloroplast-encoded genes transferred to the host's nuclear genome. Thus, in modern plant and algal cells, many nuclear-encoded chloroplast proteins synthesized in the cytosol of the cell must be imported across both the outer and inner chloroplast membranes in a process that requires energy.

In 2013, Osaka University researchers headed by Masato Nakai discovered and characterized a huge novel transport channel (TIC) in the inner chloroplast membrane through which proteins were transported (Science, 339, 571–574.) However, the motor that enables proteins to be imported across the inner membrane remained a mystery.

Now, in The Plant Cell, this same team has collaborated with other Japanese researchers to report the identification of the elusive protein transport motor that is essential for chloroplast formation.

Figure 2. The chloroplast import motor was derived from the membrane-bound protease of an endosymbiotic cyanobacterial-like ancestor. Credit: Osaka University
"We identified another huge novel protein complex, twice the mass of TIC, which is made up of six related proteins with one accessory protein, and functions as the import motor in a close association with TIC," says Nakai. "Surprisingly, the six related components all evolved from an enzyme contained within the ancestral cyanobacterium-like endosymbiont that degraded unwanted proteins after extracting them from the membrane."

Although the protein breakdown role has since been lost during evolution, the extraction function has been retained to be utilized as an import motor. The team believes that simultaneous increases in the size of components of TIC and the newly identified motor occurred early in the evolution of the green algae, perhaps to improve protein import efficiency.

"These findings revolutionize the molecular model of chloroplast import, and help us understand the of plant and algal chloroplasts," explains Nakai. "This understanding could aid biotechnological improvements in the efficiency of crop photosynthesis, or the development of and algae as factories that manufacture or store proteins in their chloroplasts."

Explore further: Amoeba offers key clue to photosynthetic evolution

More information: Shingo Kikuchi et al. A Ycf2-FtsHi heteromeric AAA-ATPase complex is required for chloroplast protein import, The Plant Cell (2018). DOI: 10.1105/tpc.18.00357

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