This article has been reviewed according to Science X's editorial process and policies. Editors have highlighted the following attributes while ensuring the content's credibility:

fact-checked

peer-reviewed publication

trusted source

proofread

Key protein regulates immune response to viruses in mammal cells

Key protein regulates immune response to viruses in mammal cells
Researchers demonstrated that the processing of the protein TRBP enhances apoptosis, reducing interferon (IFN) signaling during viral infection. Credit: Tomoko Takahashi (Saitama University/The University of Tokyo) and Kumiko Ui-Tei (The University of Tokyo)

Researchers have revealed the regulatory mechanism of a specific protein that plays a key role in balancing the immune response triggered by viral infections in mammal cells. These findings could help drive the development of antiviral therapies and nucleic acid medicines to treat genetic disorders. The research is published in the journal Nucleic Acids Research.

For cells to protect themselves from viral infections, a series of immune responses typically occur, including programmed cell death called apoptosis and interferon signaling. While apoptosis is a normal process, that occurs with or without the presence of viral molecules, following a cascade of steps to end with the death of a cell—which might not sound advantageous to the host—it can help prevent the reproduction of abnormal cells, including those infected by viruses, and eliminate them from the body.

Interferons, on the other hand, are proteins produced by animal cells in response to a viral infection to protect the cell against viral attacks and prevent the virus from replicating. The regulatory mechanism of how cells maintain a balance between apoptosis and interferon response to efficiently suppress viral replication during infection, however, remained unclear.

In the current study, a team including researchers from the University of Tokyo focused on a specific protein, TRBP, which is also classified as a type of protein called an RNA silencing factor.

RNA is a nucleic acid, an organic compound found in living cells and viruses, which controls and the genetic makeup of many viruses. RNA synthesizes proteins through a process known as translation, by reading genetic sequences and translating them into instructions for the cells to create proteins, which are mostly responsible for the overall structure and function of the organism, whether it's a plant or animal.

RNA silencing, also known as RNA interference, is a way that plants and invertebrate animals can protect themselves from viruses by cleaving viral RNA to repress viral replication.

"This study provides a significant insight that clearly revealed the protein related to the RNA silencing mechanism, which is known to be an antiviral mechanism in a plant or invertebrate, is strongly related to antiviral response also in mammals by another mechanism," said co-author Tomoko Takahashi, a visiting researcher at the University of Tokyo and assistant professor at Saitama University in Japan.

Though it is widely understood that RNA silencing is a mechanism that operates under normal conditions to control (if the gene is "turned on" to provide instructions for the cell to assemble the it encodes), it's still unclear how this process occurs under the stress of viral infection.

Key protein regulates immune response to viruses in mammal cells
Researchers revealed the regulatory mechanism of a specific protein, TRBP, that plays a key role in balancing the immune response triggered by viral infections in mammal cells. Credit: Tomoko Takahashi (Saitama University/The University of Tokyo) and Kumiko Ui-Tei (The University of Tokyo)

So the researchers looked at TRBP (an abbreviation for TAR RNA-binding protein), which has shown a significant role in RNA silencing during a viral infection.

This protein interacts with a virus sensor protein early on in the phases of infection in human cells. In the later stages of viral infection, proteins called caspases are activated, and this type of protein is chiefly responsible for triggering cell death.

"RNA silencing and interferon signaling were previously considered as independent pathways, but multiple reports, including ours, have demonstrated crosstalk between them," said Kumiko Ui-Tei, another co-author and associate professor from the University of Tokyo (at the time of the study).

This functional conversion of TRBP triggered by viral infection is the basis of regulating interferon response and apoptosis, with TRBP irreversibly increasing the programmed cell death of infected cells, while reducing interferon signaling. TRBP works on the cell by inducing , stopping the viral replication entirely, in contrast to the interferon response pathway, which just subdues instead of eliminating the infected cells.

"The ultimate goal is understanding the underlying the antiviral defense system, orchestrated through the interplay between internal and external RNA pathways in human cells," said Takahashi.

By gaining a deeper understanding of how defenses against viruses work on a molecular level, the researchers aim to drive the development of nucleic acid medicines. These medicines utilize targeting and inhibition approaches similar to the antiviral response of RNA silencing, and they hold the promise of being increasingly useful in treating a wider range of patients afflicted with , genetic mutations and genetic defects.

This study was conducted in collaboration with Saitama University, Chiba University, Kyoto University and Maebashi Institute of Technology in Japan.

More information: Caspase-mediated processing of TRBP regulates apoptosis during viral infection, Nucleic Acids Research(2024). DOI: 10.1093/nar/gkae246

Journal information: Nucleic Acids Research

Citation: Key protein regulates immune response to viruses in mammal cells (2024, April 18) retrieved 29 May 2024 from https://phys.org/news/2024-04-key-protein-immune-response-viruses.html
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.

Explore further

Discovery of how COVID-19 virus replicates opens door to new antiviral therapies

20 shares

Feedback to editors