A Sudoku-solving algorithm holds promise for protein medicine

A Sudoku-solving algorithm holds promise for protein medicine
ProteinSolver can compute novel protein sequences that fold into predetermined geometrical structures as seen in this example where the structure of the reference protein (white) is overlaid with a structure produced by a new protein sequence (blue). Credit: Alexey Strokach

Computational biologists at the University of Toronto have developed an artificial intelligence algorithm that has the potential to create novel protein molecules as finely tuned therapeutics.

The team led by Philip M. Kim, a professor of molecular genetics and computer science at the Donnelly Centre for Cellular and Biomolecular Research at U of T's Faculty of Medicine, have developed ProteinSolver, a graph that can design a fully new to fit a given geometric shape. The researchers took inspiration from the Japanese number puzzle Sudoku, whose constraints are conceptually similar to those of a .

Their findings are published in the journal Cell Systems.

"The parallel with Sudoku becomes apparent when you depict a protein molecule as a network," says Kim, adding that the portrayal of proteins in graph form is standard practice in computational biology.

A newly synthesized protein is a string of amino-acids, stitched together according to the instructions in that protein's gene code. The amino-acid polymer then folds in and around itself into a three-dimensional molecular machine that can be harnessed for medicine.

A protein converted into a graph looks like a network of nodes, representing amino-acids, connected by edges, which are the distances between them within the molecule. By applying principles from graph theory, it then becomes possible to model the molecule's geometry for a specific purpose to, for example, neutralize an invading virus or shut down an overactive receptor in cancer.

Proteins make good drugs thanks to three-dimensional features on their surface with which they bind cellular targets with more precision than the synthetic small molecule drugs that tend to be broad spectrum and can lead to harmful off-target side effects.

Just over a third of all medications approved over the last couple of years were proteins, which also make up the vast majority of top ten drugs globally, Kim said. Insulin, antibodies and growth factors are only some examples of injectable cellular proteins, also known as biologics, already in use.

Designing proteins from scratch remains incredibly difficult however, owing to the vast number of possible structures to choose from.

"The main problem in protein design is that you have a very large search space," says Kim, referring to the many ways in which the 20 naturally occurring amino-acids can be combined into protein structures.

"For a standard-length protein of 100 amino-acids, there are 20100 possible molecular structures, that's more than the number of molecules in the universe," he says.

Kim decided to turn the problem on its head, by starting with a three-dimensional structure and working out its amino-acid composition.

"It's the protein design, or the inverse protein folding problem—you have a shape in mind and you want a sequence (of amino-acids) that will fold into that shape. Solving this is in some ways more useful than protein folding, as you can in theory generate new proteins for any purpose," says Kim.

That's when Alexey Strokach, a Ph.D. student in Kim's lab, turned to Sudoku, after learning in a class about its relatedness to molecular geometry.

In Sudoku, the goal is to find missing values in a sparsely filled grid by observing a set of rules and the existing number values.

Individual amino-acids in a protein molecule are similarly constrained by their neighbors. Local electrostatic forces ensure that amino-acids carrying opposite electric charge pack closely together while those with the same charge are pulled apart.

Strokach first built the constraints found in Sudoku into a neural network algorithm. He then trained the algorithms on a vast database of available protein structures and their amino-acid sequences from across the tree of life. The goal was to teach the algorithm, ProteinSolver, the rules, honed by evolution over millions of years, of packing amino-acids together into smaller folds. Applying these rules to the engineering process should increase the chances of having a functional protein at the end.

The researchers then tested ProteinSolver by giving it existing protein folds and asking it to generate amino-acid sequences that can build them. They then took the novel computed sequences, which do not exist in nature, and manufactured the corresponding protein variants in the lab. The variants folded into the expected structures, showing that the approach works.

In its current form, ProteinSolver is able to compute novel amino-acid sequences for any protein fold known to be geometrically stable. But the ultimate goal is to engineer novel protein structures with entirely new biological functions, as new therapeutics, for example.

"The ultimate goal is for someone to be able to draw a completely new protein by hand and compute sequences for that, and that's what we are working on now," says Strokach.

The researchers made ProteinSolver and the code behind it open source and available to the wider research community through a user-friendly website.

Journal information: Cell Systems

Citation: A Sudoku-solving algorithm holds promise for protein medicine (2020, September 23) retrieved 14 June 2024 from https://phys.org/news/2020-09-sudoku-solving-algorithm-protein-medicine.html
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.

Explore further

New structural unit simplifies the process of custom-designing proteins


Feedback to editors