Removing belly fat before it sticks to you

Removing belly fat before it sticks to you                 March 18, 2020Researchers at the University of C
Sean Davidson, professor in the UC College of Medicine, is shown in his laboratory. Credit: University of Cincinnati

Triglycerides, those fats that seem to be the bane of any diet, remain a mystery for many researchers. Plenty of them are in Big Macs, deep pan pizza and the like, but some are a necessity to fuel the body for daily activities.

Researchers Mark Castleberry, a doctoral student, and professor Sean Davidson, both in the UC College of Medicine, have found a way to produce in the laboratory a produced in the liver known as APOA 5. It plays an important role in metabolizing and clearing excess levels of from the bloodstream.

Their findings are available in the American Society for Biochemistry and Molecular Biology's Journal of Lipid Research online. Castleberry, who is studying in the UC Department of Molecular Genetics, Biochemistry and Microbiology, is the paper's first author. He is shown in the photo above with Davidson.

"We are really interested in understanding triglycerides because hypertriglyceridemia—too much fat in your blood—is a big factor leading to , diabetes, obesity and other ," explains Davidson, who holds appointments in UC's departments of Pathology and Laboratory Medicine and Molecular Genetics, Biochemistry and Microbiology. "When you have a lot of fat that is hanging around in your circulation it's important to clear as much of it out as soon as possible."

"APOA5 is highly involved in how fast triglycerides get taken out of your circulation," says Davidson, who has a doctorate in . "The more APOA5 you have the faster the triglyceride is removed. Everybody agrees it is an important protein but scientists don't know much about its structure or how it does what it does. If we could figure out how it works we could come up with a drug that uses the same mechanism or trigger it to work better."

Castleberry says researchers inserted a coded by DNA into bacteria genetically engineered to produce human proteins. Once those proteins were produced they were removed from the host and purified for use in studies at the lab bench and in mouse models.

"We can quickly make a much greater amount of this protein using bacterial production than if we tried to isolate it from blood in humans," explains Castleberry. "The mice in this study were basically fed a large bowl of fat and triglycerides."

"We could analyze their blood after we fed them and observe the level of fat change as they digested the meal," said Castleberry. "We were able to give our to the mice that had that fatty meal and rapidly clear the triglycerides that would have accumulated in their blood."

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More information: Mark Castleberry et al. Functional recombinant apolipoprotein A5 that is stable at high concentrations at physiological pH, Journal of Lipid Research (2019). DOI: 10.1194/jlr.D119000103
Journal information: Journal of Lipid Research

Citation: Removing belly fat before it sticks to you (2020, March 19) retrieved 3 April 2020 from
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