Physical forces affect bacteria's toxin resistance, study finds

bacteria
Credit: CC0 Public Domain

A random conversation between two Cornell researchers at a child's birthday party led to a collaboration and new understanding of how bacteria resist toxins, which may lead to new tools in the fight against harmful infections.

Physical forces have been known to affect how cells in our body grow and survive, but little has been understood about the role these forces play in prokaryotes—single-cell organisms, including bacteria.

Christopher Hernandez, associate professor in the Sibley School of Mechanical and Aerospace Engineering, had an idea for a that would subject individual bacteria to known amounts of force and mechanical deformation. But he knew of few ways to measure the effects—until a chance encounter with Peng Chen, the Peter J.W. Debye Professor in the College of Arts and Sciences' Department of Chemistry and Chemical Biology.

Chen had developed a way to tag and observe a specific molecule that pumps toxins from the inner membrane of certain bacteria. By putting their ideas together, the researchers have shown conclusively that mechanical stresses can interrupt the ability of bacteria to survive exposure to toxins.

Their paper, "Mechanical Stress Compromises Multicomponent Efflux Complexes in Bacteria," published Nov. 26 in the Proceedings of the National Academy of Sciences.

Gram-negative bacteria are characterized by their dual-membrane cell envelope and have the ability to assemble molecular pumps to rid themselves of toxic substances that manage to migrate into the cell, including antibiotics.

Hernandez and Chen's research showed that when E. coli bacteria were placed into a microfluidic device and forced to flow into very tight spaces, the resulting alone were enough to cause these pumps to break apart and stop working.

"This is one of the first studies to look at the mechanobiology of bacteria," Hernandez said. "Our findings provide evidence that bacteria are similar to other types of in that they respond to mechanical forces through molecular complexes."

"Our work shows that you can disrupt the pump complex of with mechanical means," Chen said, "and this may give us a new tool to enhance treatments of bacterial diseases."

The methodology Hernandez and Chen created can be used to examine all sorts of prokaryotic cell structures, functions and behavior.

"This creative, collaborative research effort, which exploits capabilities in single molecule biology, will provide the Army with a better fundamental understanding of not only the cellular features that keep microbes alive, but how mechanical stress at the cellular level can control bacterial viability and thus provide a novel potential means of controlling bacterial infections," said Robert Kokoska, program manager for microbiology at Army Research Office, an element of U.S. Army Combat Capabilities Development Command's Army Research Laboratory that supported the research.


Explore further

Bacteria must be 'stressed out' to divide

More information: Lauren A. Genova et al. Mechanical stress compromises multicomponent efflux complexes in bacteria, Proceedings of the National Academy of Sciences (2019). DOI: 10.1073/pnas.1909562116
Provided by Cornell University
Citation: Physical forces affect bacteria's toxin resistance, study finds (2019, December 5) retrieved 28 January 2020 from https://phys.org/news/2019-12-physical-affect-bacteria-toxin-resistance.html
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
305 shares

Feedback to editors

User comments