Turning a dangerous toxin into a biosensor

Turning a dangerous toxin into a biosensor
Credit: Chan Cao, EPFL

Some types of bacteria have the ability to punch holes into other cells and kill them. They do this by releasing specialized proteins called "pore-forming toxins" (PFTs) that latch onto the cell's membrane and form a tube-like channel that goes through it. This structure across the membrane is called a pore. Punctured by multiple PFTs, the target cell self-destructs.

However, PFTs have garnered much interest beyond bacterial infections. The nano-sized pores that they form are used for sensing biomolecules: A , e.g., DNA or RNA, passes through the nanopore like a string steered by a voltage, and its individual components (e.g., in DNA) give out distinct electrical signals that can be read out. In fact, nanopore sensing is already on the market as a major tool for DNA or RNA sequencing.

Publishing in Nature Communications, scientists led by Matteo Dal Peraro at EPFL have studied another major PFT that can be used effectively for more complex sensing, such as protein sequencing. The toxin is aerolysin, which is produced by the bacterium Aeromonas hydrophila, and is the "founding member" of a major family of PFTs found across many organisms.

One of the main advantages of aerolysin is that it forms very narrow pores that can tell apart molecules with much higher resolution than other toxins. Previous studies have shown that aerolysin can be used to "sense" several biomolecules, but there have been few studies on the relationship between aerolysin's structure and its molecular sensing abilities.

Molecular simulation of an engineered aerolysin pore (light blue color) embedded into a membrane bilayer (cream color) and translocating DNA (red color). Credit: Chan Cao, EPFL

The researchers first used a structural model of aerolysin to study its structure with computer simulations. As a protein, aerolysin is made up of , and the model helped the scientists understand how those amino acids affect the function of aerolysin in general.

Once they had a grasp of that relationship, the researchers strategically changed amino acids in the computer model. The model then predicted the possible impact of each change on the overall function of aerolysin.

At the end of the computational process, Dr. Chan Cao, the leading author of this work, produced 16 genetically engineered, "mutant" aerolysin pores, embedded them in to simulate their position in a cell membrane, and carried out various measurements (single-channel recording and molecular translocation experiments) to understand how ionic conductance, ion selectivity, and translocation properties of the aerolysin are regulated on a molecular level.

And with this approach, the researchers finally found what drives the relationship between the structure and the function of aerolysin: its cap. The aerolysin pore isn't just a tube that goes through the membrane, but also has a cap-like structure that attracts and tethers the target molecule and "pulls" it through the pore's channel. And the study found that it is the electrostatics at this cap region that dictate this relationship.

"By understanding the details of how the of the aerolysin pore connects to its function, we can now engineer custom pores for various sensing applications," says Dal Peraro. "These would open new, unexplored opportunities to sequence biomolecules as DNA, proteins and their post-translational modifications with promising applications in gene sequencing and biomarkers detection for diagnostics." The scientists have already filed a patent for their sequencing and characterization of the genetically engineered aerolysin pores.


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More information: Chan Cao, Nuria Cirauqui, Maria Jose Marcaida, Buglakova Elena, Alice Duperrex, Aleksandra Radenovic, Matteo Dal Peraro. Single-molecule sensing of peptides and nucleic acids by engineered aerolysin nanopores. Nature Communications 29 October 2019. DOI: 10.1038/s41467-019-12690-9
Journal information: Nature Communications

Citation: Turning a dangerous toxin into a biosensor (2019, October 29) retrieved 19 November 2019 from https://phys.org/news/2019-10-dangerous-toxin-biosensor.html
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