Massive database traces mammal organ development, cell by single cell

February 20, 2019, Allen Institute for Brain Science
Credit: CC0 Public Domain

The very early days of growth, long before we are born, are a time of incredible development. In a relatively short period of time, we and other mammals create our bodies' dozens of different organs from a few thin layers of cells. In mice, that period is only four days long. In humans, it's complete before the end of the first trimester of pregnancy.

Now, a new study by researchers at the Allen Discovery Center at UW Medicine has traced that important period of organ formation, cell by cell, in the developing mouse. Published today in the journal Nature, the study is by far the largest dataset of its kind to date.

The study captured the that switch on and off across 2 million different as they go from undistinguished cellular precursors to becoming the animals' stomach, muscles, brain, skin and everything else in between.

Understanding how we grow from one cell into thousands of different types of cells, all connected and functioning together to make our adult bodies, is essential to understanding not only human biology, but much of life itself, said Jay Shendure, M.D., Ph.D., a senior author on the study and leader of the Allen Discovery Center.

"We each came from a single cell—not just every human, but every multicellular organism on the planet. These cell lineages result in us becoming functioning organisms, but are also what unites us," said Shendure, who is also an Investigator of the Howard Hughes Medical Institute and Professor of Genome Sciences at the University of Washington. "Major subsets of the tree of life share this general developmental program."

Development is also at the foundation of many human diseases, even those that don't manifest until much later in life.

"Not only developmental diseases, but myriad common diseases of adulthood have some root in processes of , and we just don't understand those things well enough yet," Shendure said.

Understanding our genetic program

The study relied on a new technique developed by Junyue Cao, a UW graduate student working in the Allen Discovery Center and co-lead author on the paper, for measuring how genes are turned on and off, also known as , from individual cells across all parts of the animals' bodies and different stages of development. The researchers labeled the output of each cell's genes with a unique set of three molecular barcodes, which are then read out at the same time as the rest of the cell's gene expression data.

This triple-labeling means the researchers can mix many cells together in one test tube to capture their gene expression and still trace those gene products back to a single cell, thanks to their individual barcodes. It's the reason why Cao could run this two-million-cell experiment in under two weeks, but the technique development itself took close to a year—as did the analysis of the resulting pile of data.

Cao, together with Malte Spielmann, Ph.D., a former UW postdoctoral fellow, co-first author on the paper and now faculty at the Max Planck Institute for Molecular Genetics, used this method to study the gene expression of single cells from 61 lab mouse embryos of different ages across that four-day window of development. Their analysis didn't capture every in these embryos, but from the researchers' estimates, they got pretty close in some of the developmental stages. They were able to study about 80 percent of cells in the earliest embryos and between 3 and 20 percent in the slightly older embryos.

This work is very different from classical methods of studying developmental biology, where researchers would mutate a single gene or a few genes and see what structures changed in the resulting developing animals.

"That approach only gives you a glimpse into this underlying genetic architecture of development," said Cole Trapnell, Ph.D., an Assistant Professor of Genome Sciences at UW and a member of the Allen Discovery Center at UW Medicine. Trapnell was also a senior author on the study. "If you could watch the entire process unfold at incredibly high resolution and then apply sophisticated computer algorithms to organize the data, you might be able to map out much bigger pieces of the genetic program that control development," he said.

This study isn't quite at that point yet. The researchers didn't track cells in the same animal as they developed, although that kind of lineage tracing is one of their long-term goals. But it's a step on that path and could still yield valuable information about the biology of development, the researchers said.

In their study, they delved into a few key types of development, namely the formation of limbs and of skeletal muscle, two general processes that are very similar in mice and humans. The researchers found hundreds of genes switching on and off in brief time periods in the specific cells that drive leg development, genes that hadn't been linked to these cells in previous studies. They don't yet know what those genes are doing, and there's much more yet to uncover in this dataset, they said.

"It's going to take a whole community of researchers years to look at this data to the point where we feel like we've exhausted it," Trapnell said. "We're really only scratching the surface of what this data will mean for the field."

Explore further: Discovery in cell development changes understanding of how genes shape early embryos

More information: The single-cell transcriptional landscape of mammalian organogenesis, Nature (2019). DOI: 10.1038/s41586-019-0969-x , https://www.nature.com/articles/s41586-019-0969-x

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FredJose
2.3 / 5 (3) Feb 21, 2019
Strange how people look straight into the most sophisticated programs ever seen by mankind and then come to the conclusion that it happened by accident.
There is NOTHING ever invented by even the most intelligent human beings on this planet that comes close to what we observe in the development of a living organism from the time that the gestation has started. Yet these same supposedly intelligent beings rigidly adhere to the false idea that such complexity can happen all by itself via random chemical, biological and physical processes with no outside intelligent and all-powerful help. That is just plain idiotic.
According to these intelligent humans, purely MATERIAL based processes are responsible for generating ABSTRACT information - producing sophisticated signalling coding and decoding mechanisms all by itself. How that happens is the greatest of impossible miracles, but they will insist.
The book of life (DNA) wrote itself, they say, just like a Shakespearean novel.
Da Schneib
3 / 5 (2) Feb 21, 2019
FreddyJoe thinks jebus did it.

Here you go, @FreddyJoe, this is what you worship: https://pbs.twimg...pg:large

Go spit in the oil barrel fire in the trailer park. Then you can make a comment about "the damn libruls."

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