Increased cyto-adhesion of malaria parasites during fever uncovered

Increased cyto-adhesion of malaria parasites during fever uncovered
Microscopic images of adhesion studies between a plasmodium-infected RBC & CHO cell. Credit: SUTD

Malaria is the most prevalent blood-borne infectious disease caused by parasites of the species Plasmodium. In 2016, more than 216 million malaria infections were reported resulting in 445,000 deaths across the developing world.

During the blood stage development, Plasmodium (P.) falciparum, the most common causative agent of malaria-associated pathology in humans, exports a number of parasitic proteins to the surface of infected facilitating its cyto- to endothelial receptors. This adhesive behavior prevents splenic clearance and induces microvasculature obstruction, forming critical contributors towards the disease outcome.

In a recent study, a research team from Singapore demonstrated how the adhesion of -infected blood cells is elevated at febrile temperatures. Through dual-micropipette step-pressure technique between P. falciparum-infected RBCs (iRBCs) and Chinese Hamster Ovary cells expressing Chondroitin sulfate A (CHO-CSA), they determined that adhesion is elevated at febrile temperatures. The team also noticed that exposures to febrile temperature significantly increased both the adhesion force and adhesion percentage between iRBCs and CSA-CHO .

Using flow cytometry analysis, the team documented an increase in phosphatidylserine expression on the iRBC surface following exposure to febrile . They demonstrate that elevated levels of phosphatidylserine is linked to increased cyto-adhesion, since the trend was reversed by introducing soluble Annexin V. "These results suggest that elevated PS recruitment on iRBC under thermally stressed conditions contributes to the increased adhesive behavior of iRBCs, which might be relevant to clinical manifestations associated with malaria fever," said Dr. Rajesh Chandramohanadas from the Singapore University of Technology & Design (SUTD).

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More information: Rou Zhang et al, Febrile Temperature Elevates the Expression of Phosphatidylserine on Plasmodium falciparum (FCR3CSA) Infected Red Blood Cell Surface Leading to Increased Cytoadhesion, Scientific Reports (2018). DOI: 10.1038/s41598-018-33358-2
Journal information: Scientific Reports

Citation: Increased cyto-adhesion of malaria parasites during fever uncovered (2018, October 10) retrieved 1 April 2020 from
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