Was the primordial soup a hearty pre-protein stew?

September 4, 2017, Georgia Institute of Technology
The first polymers of life may have arisen by a daily process still observed on Earth today, such as the repeated drying and refilling of pond water. Credit: Ram Krishnamurthy / Center for Chemical Evolution

The primordial soup that sloshed around billions of years ago, and eventually led to first life on our planet, might have been teeming with primal precursors of proteins.

Ancestors of the first protein molecules, which are key components of all cells, could have been bountiful on pre-life Earth, according to a new study led by researchers at the Georgia Institute of Technology, who formed hundreds of possible precursor molecules in the lab. Then they meticulously analyzed the molecules with latest technology and new algorithms.

They found that the molecules, called depsipeptides, formed quickly and abundantly under conditions that would have been common on prebiotic Earth, and with ingredients that would have likely been plentiful.

And some of the depsipeptides evolved into new varieties in just a few days, an ability that, eons ago, could have accelerated the birth of long molecules, called peptides, that make up proteins.

Sans cataclysm, please

The new NASA-affiliated research adds to a growing body of evidence suggesting that the first polymers of life may have arisen in variations of daily processes still observed on Earth today, such as the repeated drying and refilling of pond water. They may not have all zapped into existence as a result of blazing cataclysms, an image often associated with the creation of the first chemicals of life.

"We want to stay away from scenarios that are not readily possible," said Facundo Fernández, a professor in Georgia Tech's School of Chemistry and Biochemistry, and one of the study's principal investigators. "Don't deviate from conditions that would have been realistic and reasonably common on prebiotic Earth. Don't invoke any unreasonable chemistry."

Scientists have long puzzled over how the very first proteins formed. Their long-chain molecules, polypeptides, can be tough to make in the lab under abiotic conditions.

Some researchers have toiled to build tiny chains, or peptides, sometimes under more extreme scenarios that probably occurred less often on early Earth. The yields have been modest, and the resulting peptides have had only a couple of component parts, whereas natural proteins have a large variety of them.

Step-by-step evolution

But complex molecules of life likely did not arise in one dramatic step that produced final products. That's the hypothesis that drives the research of Fernández and his colleagues at the NSF/NASA Center for Chemical Evolution, headquartered at Georgia Tech and based on close collaboration with the Scripps Research Institute.

Instead, multiple easier chemical steps produced plentiful in-between products that were useful in subsequent reactions that eventually led to the first biopolymers. The depsipeptides produced in this latest study could have served as such a chemical stepping stone.

They look a lot like regular peptides and can be found today in biological systems. "Many antibiotics, for example, are depsipeptides," Fernández said.

Fernández, his Georgia Tech colleagues Martha Grover and Nicholas Hud, and Ram Krishnamurthy from Scripps published their study on August 28, 2017, in the journal Proceedings of the National Academy of Sciences. First author Jay Forsythe, formerly a postdoctoral researcher at Georgia Tech, is now an assistant professor at the College of Charleston. Research was funded by the National Science Foundation and the NASA Astrobiology Program.

The new study joins similar work about the formation of RNA precursors on prebiotic Earth, and about possible scenarios for the formation of the first genes. The collective insights may someday help explain how first life arose on Earth and also aid astrobiologists in determining the probability of life existing on other planets.

Understanding depsipeptide Lego

To understand depsipeptides and the significance of the researchers' results, it's helpful to start by looking at peptides, which are chains of amino acids. When the chains get really long they are called polypeptides, and then proteins.

Living cells have machinery that reads instructions in DNA on how to link up amino acids in a specific order to build very specific peptides and proteins that have functions in a living cell. For a protein to have function in a cell, its polypeptide chains have to clump up like sticky yarn to form useful shapes.

Before cells and DNA existed on an Earth devoid of life, for polypeptides to form, amino acids had to somehow jostle together in puddles or on the banks of rivers or lakes to form chains. But peptide bonds can be tough to form, especially long chains of them.

Amino stand-in double

Other bonds, called ester bonds, form more easily, and they can link up amino acids with very similar molecules called hydroxy acids. Hydroxy acids are so much like amino acids that they can, in some cases, function as their stand-in doubles.

The researchers mixed three amino acids with three hydroxy acids in a water solution and they formed depsipeptides, chains of amino acids and hydroxy acids held together by intermittent ester and peptide bonds. The hydroxy acids acted as an enabler to put the chains together that would have otherwise been difficult to form.

The primordial soup may have lapped its depsipeptides onto rocks, where they dried out in the sun, then rain or dew dissolved them back into the soup, and that happened over and over. The researchers mimicked this cycle in the lab and watched as the depsipeptide chains further developed.

Death Valley heat

"We call it an environmental cycling approach to making these early peptides," said Fernández, who is Vasser Woolley Foundation Chair in Bioanalytical Chemistry. Like nature: Make the soup, dry it out, repeat.

In the lab, the drying temperature was 85 degrees Celsius (185 degrees Fahrenheit), although the reaction has been shown to work at temperatures of 55 and 65 degrees Celsius (131 to 149 degrees Fahrenheit). "If you think about early Earth having a lot of volcanic activity and an atmospheric mix that promoted warming, those temperatures are realistic on many parts of an early Earth," Fernández said.

Early Earth took hundreds of millions of years to cool, and temperatures in the hundreds of degrees are hypothesized to have been commonplace for a long time. Even today, the hottest deserts can reach over 55 degrees Celsius.

Ester do-si-do

Since ester bonds break more easily, in the experiment, the chains tended to come apart more at the hydroxy acids and hold together between the amino acids, which were connected by the stronger peptide bonds. As a result, chains could re-form and link up more and more amino acids with each other into sturdier peptides.

In a kind of square-dance, the stand-in hydroxy acids often left their amino partners in the chain, and new amino acids latched onto the in their place, where they held on tight. In fact, a number of the depsipeptides ended up being composed almost completely of amino acids and had only remnants of hydroxy acids.

"Now we know how can form easily," Fernández said. "Next, we want to find out what's needed to get to the level of a functional protein."

10,000,000,000,000 depsipeptides

To identify the more than 650 depsipeptides that formed, the researchers used mass spectrometry combined with ion mobility, which could be described as a wind tunnel for molecules. Along with mass, the additional mobility measurement gave the researchers data on the shape of the depsipeptides.

Algorithms created by Georgia Tech researcher Anton Petrov processed the data to finally identify the molecules.

To illustrate how potentially bountiful depsipeptides could have been on prebiotic Earth: The researchers had to limit the number of and hydroxy acids to three each. Had they taken 10 each instead, the number of theoretical depsipeptides could have climbed over 10,000,000,000,000.

"Ease and bounty are key," Fernández said. "Chemical evolution is more likely to progress when components it needs are plentiful and can join together under more ordinary conditions."

Explore further: Finding the origins of life in a drying puddle (w/ Video)

More information: Jay G. Forsythe et al. Surveying the sequence diversity of model prebiotic peptides by mass spectrometry, Proceedings of the National Academy of Sciences (2017). DOI: 10.1073/pnas.1711631114

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9 comments

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Jayarava
1.7 / 5 (6) Sep 04, 2017
"primordial soup" theories of the origins of life have been discredited. Soups do not evolve.
Jayarava
1.8 / 5 (5) Sep 04, 2017
One of the main problems with soup theories is concentration. Let's say these molecules did manage to form due to volcanic activity, they ended up in the sea. The vast primordial oceane where they would have been diluted to parts per million or more likely billion. Many different kinds perhaps, but not "bountiful", not present in the kinds of concentrations required for bio-chemistry.

By what mechanism were the molecules concentrated to the point where they could undergo polymerisation? This won't happen at ppb or ppm. Without an attempt to answer this question, the research is just meaningless.

But more to the point, where is the evidence from outside the lab that such compounds are being formed in today's volcanoes. Just because you can tweak the parameters to give you a positive result in the lad, does not mean that such conditions obtained on the earth. If such a process occurred then, it must be occurring now.
Jayarava
2 / 5 (4) Sep 04, 2017
At present the only theory that even addresses the concentration problem is the warm-alkaline undersea vents theory. Unfortunately for the soup enthusiasts it also solves several other problems, including the origin of the organic molecules necessary, the catalysts required, and the energy input making it thermo-dynamically feasible.

No other theory even gets close to this level of sophistication. More effort needs to go into testing WAUSV and filling out the gaps, rather then warming up the soup.
johnhew
5 / 5 (2) Sep 04, 2017
I would say de-emphasized rather than discredited Jay, only creditors can discredit
Steve 200mph Cruiz
3.7 / 5 (3) Sep 04, 2017
Jayarava

Your "theory" is a bunch of assumptions and a lack of understanding of o chem
syndicate_51
not rated yet Sep 04, 2017
"To illustrate how potentially bountiful depsipeptides could have been on prebiotic Earth: The researchers had to limit the number of amino acids and hydroxy acids to three each. Had they taken 10 each instead, the number of theoretical depsipeptides could have climbed over 10,000,000,000,000."

So whats the actual full on number?
yep
5 / 5 (2) Sep 05, 2017
"primordial soup" theories of the origins of life have been discredited. Soups do not evolve.

What a ridiclious statement, soups like pasta sauces can evolve to be much tastier after a day or two.
Steelwolf
not rated yet Sep 06, 2017
Jayarava,

They have already determined, for reasons you point out, that life as such starting in oceans had a low probability, wheras the concentrations needed would have happened in smaller ponds and lakes, and, as this article shows. Seasonality with dryer and wetter periods would have changed the concentration, and thus heightened the chances for these polymer chains to form.

The soup need not be an oceanful, but a nice set of ponds will do just fine.
Steelwolf
not rated yet Sep 06, 2017
Also, as far as "how many?"...to start out with there are over 200 different amino acids. We use only 20 of them in our bodies. On Hydroxy Acids, there are at least 30 different types, as a starting point and so that gives a Huge Boost to the finished numbers, and the longer the chains, the greater the number of combinations. Nearly infinite. 6 X 10 to the 16th power would actually be a conservative estimate.

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