Researchers uncover a role for HSP90 in gene-environment interactions in humans

February 23, 2017, Whitehead Institute for Biomedical Research

Proteins do most of the work inside the human body, supporting the structure, function, regulation, and repair of organs, tissues and cells. Proteins are synthesized as extended chains of amino acids that must fold into intricate three-dimensional shapes to perform their work. However, protein folding is a very delicate process: crowded conditions within a cell or environmental changes around the cell can drive proteins to misfold and clump together or aggregate—causing disease.

To support effective folding, deploy -folding chaperones to protect them from misfolding and aggregation. The heat-shock proteins 90 and 70 (HSP90 and HSP70) are protein-folding chaperones that together assist a large fraction of the proteins in cells to fold and function. In so doing, these major chaperones have been shown to alter the biological effects of in model organisms such as in plants, yeast, flies and fish. However, it has been unclear whether and how chaperones influence the consequences of genetic mutation in humans.

Researchers at the Whitehead Institute have now uncovered a role for HSP90 in humans, not only as a modifier of the effects of mutations, but as a mediator of the impact of the environment on the function of mutant proteins. And these effects of HSP90 can alter the course of human diseases. Their work is discussed in the paper, HSP90 shapes the consequences of , which appears today in Cell.

"By helping proteins fold and function, HSP90 influences a range of characteristics in simple organisms on which natural selection can act, and thus alters the course of evolution," says Georgios Karras, lead author of the study and a post-doctoral researcher at Whitehead Institute. "Our work demonstrates that HSP90 plays a similar role in humans, and in doing so it influences how disease-causing mutations manifest within cells and in the clinic. We found that HSP90 exerts a protective role on the functions of mutant proteins it directly binds, buffering the detrimental effects of mutations they carry."

While HSP90 may buffer the effect of a mutation, its ability to do so is highly susceptible to environment changes. "HSP90 evolved to cope with environmental stressors that perturb the folding of proteins within cells," says Luke Whitesell, a senior author of the paper and a senior scientist at Whitehead Institute. "Yet, because HSP90's capacity is limited it can also make the mutant protein much more sensitive to environmental challenges. We observed that even mild stresses, such as fever, can provoke major effects in cells expressing HSP90-buffered mutants. This finding may help to explain why mutations can have varied clinical manifestations from person to person."

Nevertheless, HSP90 cannot buffer all mutations. Some cause such problems that they cannot be rescued and these tend to be recognized by another chaperone, HSP70. Thus, the severity of the mutations' effects are reflected in the pattern of HSP90 vs. HSP70 binding to the .

"This pattern fits well with the biochemical roles of HSP90 and HSP70 during protein-folding," Karras notes. "HSP70 binds extended stretches of that are typically hidden in the core of the normally folded protein, while HSP90 binds to partially folded proteins. A severe mutation that leads to major unfolding and loss-of-function mainly drives HSP70 binding greater than HSP90 binding. Milder mutations have the opposite effect. Based on this pattern, we estimate that HSP90 can influence the consequences of up to 25 percent of missense mutations in some genes."

The study was based on data mined from more than 1,500 disease-causing associated with a diverse spectrum of diseases, including the cancer-predisposing syndrome Fanconi Anemia.

In the long run, this new understanding of within cells could help clinicians predict who is at risk to develop a particular disease and who is most likely to respond well to specific treatment approaches.

Explore further: Dangerous mistaken identity: Chaperone binds protein responsible for Alzheimer's disease

More information: HSP90 shapes the consequences of human genetic variation, Cell, February 23, 2017

Related Stories

Protein folding becomes cancer treatment target

December 3, 2013

(Medical Xpress)—A molecule that helps cancer cells to keep dividing could be a promising target for new treatments, according to research published in the journal Oncogene.

A single therapy slows multiple cancers

January 23, 2012

Targeting a single protein can help fight both breast cancers and leukemias, according to two reports published online on January 23 in the Journal of Experimental Medicine.

Shedding light on an assistant protein

June 20, 2016

Observing in-protein motions with high spatial and temporal resolution: This is made possible by a new technology developed by scientists from the University of Würzburg, giving new insight into the functional mechanisms ...

Recommended for you

Coffee-based colloids for direct solar absorption

March 22, 2019

Solar energy is one of the most promising resources to help reduce fossil fuel consumption and mitigate greenhouse gas emissions to power a sustainable future. Devices presently in use to convert solar energy into thermal ...

EPA adviser is promoting harmful ideas, scientists say

March 22, 2019

The Trump administration's reliance on industry-funded environmental specialists is again coming under fire, this time by researchers who say that Louis Anthony "Tony" Cox Jr., who leads a key Environmental Protection Agency ...


Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.