Three-photon microscopy improves biological imaging

January 22, 2013 by Anne Ju
Three-photon microscopy improves biological imaging
A horizontal frame from 3-D reconstructed three-photon microscopy images in a mouse brain. The red is neurons, the blue is blood vessels, and the dark holes are non-fluorescent neurons. Credit: Xu lab

(Phys.org)—Scientists may be a step closer to cracking one of the world's most compelling mysteries: the impossible complexity of the brain and its billions of neurons. Cornell researchers have demonstrated a new way of taking high-resolution, three-dimensional images of the brain's inner workings through a three-fold improvement in the depth limits of multiphoton microscopy, a fluorescence-based imaging technique with Cornell roots.

Publishing in the journal Jan. 20, senior author Chris Xu, associate professor of applied and , and colleagues have demonstrated high-resolution, 3-D imaging of the subcortical region of a live, intact mouse brain.

They have broken the fundamental depth limit of standard two-photon microscopy, which is a widely used imaging technology invented in 1990 by Watt Webb and Winfried Denk at Cornell. Xu and Webb demonstrated three-photon fluorescence imaging while Xu was a graduate student in Webb's lab in 1995, but its advantages were not fully recognized then, Xu said.

The new paper describes three-photon fluorescence, combined with a longer excitation wavelength of the laser pulse, to overcome such obstacles as tissue scattering and absorption, which prohibit high-resolution imaging deep within . The new paper shows that three-photon microscopy is a much better technology in the context of deep tissue imaging, making multiphoton truly "multiphoton."

Using a , the researchers have proved the principle of three-photon microscopy operating at a wavelength of 1,700 nanometers. This, in combination with the new laser specifically created for three-photon excitation, allows the researchers to perform high-resolution imaging of neurons at unprecedented depths within a mouse brain.

A 3-D reconstruction of three-photon microscopy images in a mouse brain, which contains red fluorescent protein-labeled neurons. Credit: Xu lab

Pushing these depth limits is important for basic science and eventually could prove useful clinically, Xu said. Depression and diseases like Parkinson's and Alzheimer's are associated with changes deep inside the brain, and finding the cures could be helped by subcortical neural imaging—that is, below the gray matter of the brain and into the white matter and beyond, if the brain is visualized as stacked layers.

"Brain mapping could be the so-called grand challenge within the next decade," Xu said. "With MRI, we can see the whole brain but not with the resolution we have demonstrated. The optical resolution is about 100 to 1,000 times higher and allows us to clearly visualize individual neurons."

In the mouse model, the researchers used dyes and transgenic mice to test their multiphoton microscope on different fluorescent signals and prove their concept. If three- can be used to map the entire , it could ultimately help shed light on the functions of human brains and pave the way to breakthroughs in neuroscience and other clinically relevant areas, Xu said.

Explore further: Phosphorescence mapping provides high-speed 3-D images in living tissue

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vacuum-mechanics
2.3 / 5 (3) Jan 22, 2013
They have broken the fundamental depth limit of standard two-photon microscopy, which is a widely used imaging technology invented in 1990 by Watt Webb and Winfried Denk at Cornell. Xu and Webb demonstrated three-photon fluorescence imaging while Xu was a graduate student in Webb's lab in 1995, but its advantages were not fully recognized then, Xu said.

By the way talking about photon, it is interesting to note that up to now we still do not know what really 'photon' is and how it was created! This problem once was asked by Feynman's (Noble Prize winner) father and he could not answer it! May understand the mechanism (below) could help us know better about muti-photon works.
http://www.vacuum...21〈=en

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