First close up images of Chalara fraxinea growing on the leaf stem of infected ash

May 14, 2013
First close up images of Chalara fraxinea growing on the leaf stem of infected ash

The images were obtained using cryo scanning electron microscopy, where the sample is plunged into liquid nitrogen to freeze it and imaged using the electron microscope.

The benefit of this method is that the sample is imaged in as close to its natural state as possible, providing the best quality 3D view of an organism.

First close up images of Chalara fraxinea growing on the leaf stem of infected ash

We want to understand how the fungus makes the fruiting bodies (toadstools) that produce the spores called ascospores which cause infection of ash. The fungus growing within the ash leaf stem produces and that the images show different stages of growth of such an organ. We think that this organ may be fertilised to initiate the sexual cycle that produces infective ascospores.

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