Genetic activity in the entire genome of multicellular fungi analysed at a stroke

October 1, 2012
The fungus Sordaria macrospora forms round immature fruiting bodies (left). After laser microdissection, holes are seen at the site where previously the fruiting bodies were located (right). © Ines Teichert, Gabriele Wolff, Ulrich Kück, Minou Nowrousian

With a combination of microscopic laser scissors and modern sequencing methods, biologists at the Ruhr-Universität have analyzed the activity of genes in the entire genome of certain fungi in one fell swoop. Especially with organisms in the millimetre size range, it is a particular challenge because little cell material is available. The scientists of the RUB Department of General and Molecular Botany took advantage of the method to investigate the development of small multicellular fungi.

The results are reported in the journal .

Gene activity differs from tissue to tissue

In multicellular organisms, each cell contains the same , however, often only a fraction of the genes are active (expressed). These differences in are the cause of variations in the structure and physiology of cells. Gene expression is therefore the key to understanding the development of . "In large organisms such as plants, it is usually not a problem to get enough starting material to study gene expression," explains Dr. Minou Nowrousian. "In the case of microorganisms, organs often consist of only a few cells, and might be embedded in other tissues from which they are difficult to separate." Therefore, biologists of the research groups of Prof. Dr. Ulrich Kück and Minou Nowrousian combined laser microdissection with modern to analyze the gene activity during the development of certain just 0.5 millimetres large sexual structures of fungi.

How laser microdissection works

In laser microdissection, scientists cut defined regions of a sample under the with a laser beam. With this laser mini-scissors, the RUB researchers collected the fruiting bodies, i.e. the sexual structures of the fungus Sordaria macrospora, which has been used for decades as a in . From the fruiting bodies, they isolated the RNA which represents the . With the help of "next generation" sequencing, they characterized the activity of all genes of the genome simultaneously.

A transcription factor controls genetic activity in young fruiting bodies

The Bochum researchers compared the wild-type fungus with a mutant form that has no mature fruiting bodies, in other words is not able to reproduce sexually. For this purpose, they studied gene expression in young, immature fruiting bodies. They showed that some fruiting body-specific genes are not activated in the mutant. The defective gene contains the "building instructions" for a so-called transcription factor - a protein that turns other genes on or off. The RUB team also found that the fruiting body has a completely different genetic activity pattern to non-reproductive tissue. "With the new combination of methods, we want to investigate the activity of genes in other mutants and developmental stages to better understand the molecular mechanisms of multicellular development in fungi," said Prof. Kück.

Fungi: ecological and economic importance

Fungi have a big impact on virtually all ecosystems. They make significant contributions to the reduction of animal and vegetable waste products and thereby contribute to the global carbon cycle. Some species live in symbiosis with plants or animals, other species are pathogens. In the chemical and pharmaceutical industry, fungi are used for the production of antibiotics and enzymes. The formation of pathogenic or symbiotic interactions and the production of medicines or biotechnology-related substances are often tied to specific stages in the life cycle of a fungus. The analysis of the development is therefore crucial not only for basic research but also for industrial applications.

Explore further: Good preparation is key -- even for plant cells and symbiotic fungi

More information: I. Teichert, G. Wolff, U. Kück, M. Nowrousian (2012): Combining laser microdissection and RNA-seq to chart the transcriptional landscape of fungal development, BMC Genomics, doi: 10.1186/1471-2164-13-511

Related Stories

New method for studying gene activity developed

November 14, 2011

( -- Researchers from UQ's Institute for Molecular Bioscience (IMB), Harvard University and RocheNimblegen Inc. have developed a new method for examining genetic information that reveals clues to understanding ...

An sRNA controls a bacterium's social life

May 20, 2010

For the first time, biologists have directly shown how spontaneous mutation of a small RNA (sRNA) regulatory molecule can provide an evolutionary advantage. Reporting in this week's Science, Indiana University Bloomington ...

GM safety debate may have new twist

October 28, 2010

By studying plant-fungi-bacteria interactions at plant wound sites, the team have identified a natural process stimulated by a hormone released by the wounded plant that would allow synthetic genes to move across organisms ...

Tracking genes' remote controls

January 9, 2012

As an embryo develops, different genes are turned on in different cells, to form muscles, neurons and other bodily parts. Inside each cell's nucleus, genetic sequences known as enhancers act like remote controls, switching ...

Recommended for you

Mammal long thought extinct in Australia resurfaces

December 15, 2017

A crest-tailed mulgara, a small carnivorous marsupial known only from fossilised bone fragments and presumed extinct in NSW for more than century, has been discovered in Sturt National Park north-west of Tibooburra.

Finding a lethal parasite's vulnerabilities

December 15, 2017

An estimated 100 million people around the world are infected with Strongyloides stercoralis, a parasitic nematode, yet it's likely that many don't know it. The infection can persist for years, usually only causing mild symptoms. ...


Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.