This article has been reviewed according to Science X's editorial process and policies. Editors have highlighted the following attributes while ensuring the content's credibility:

fact-checked

peer-reviewed publication

trusted source

proofread

Observing mammalian cells with superfast soft X-rays

Observing mammalian cells with superfast soft X-rays
The cells were placed in a culture medium within a vacuum-sealed holder. On the left are images captured by a single shot of around 30 femtoseconds, and on the right the same cell captured shortly after through a composite of 15 shots over a total of 250 milliseconds. The second image shows the darker carbon-enriched regions with less noise, but the researchers note that it is more blurred, probably because of cellular movement. Credit: Optica (2024). DOI: 10.1364/OPTICA.515726

Researchers have developed a new technique to view living mammalian cells. The team used a powerful laser, called a soft X-ray free electron laser, to emit ultrafast pulses of illumination at the speed of femtoseconds, or quadrillionths of a second.

With this they could capture images of carbon-based structures in living cells for the first time, before the soft X-ray radiation damaged them. Refined Wolter mirrors, a type of ultraprecise mirror, were created to enable the microscope to capture images with high spatial resolution and a wide field of view.

In the future, the team hopes to use this microscope to better understand the dynamic nature of cellular biology. The study is published in the journal Optica.

There's a difference between soft X-rays and hard X-rays. Hard X-rays are what you'll most likely have encountered if you've been through airport security or suffered a broken limb. Soft X-rays are more typically restricted to research, from studying biology and chemistry to minerals and meteorites. Soft X-rays are able to provide chemical information about samples and detailed images at the subcellular level, but their use has been limited due to the very specialized equipment required and, in biology, the damage they cause to living cells.

However, a team of researchers has constructed a new soft X-ray microscope through which they could view live for the first time. They were able to take images of carbon structures within the cells, which had not been seen before through other instruments. Carbon is one of the main elements of life, so this provides a new window into a vital part of ourselves.

The microscope has two key components: a soft X-ray free electron laser; and highly precise Wolter mirrors, a type of mirror widely used in X-ray telescopes for observing space. The mirrors were made using technology created by lead author Satoru Egawa, assistant professor of the Research Center for Advanced Science and Technology at the University of Tokyo.

Observing mammalian cells with superfast soft X-rays
In this schematic, the condenser and objective (which look like two cones here) are the Wolter mirrors. The light reflected off the second Wolter mirror forms the shadow image of the cell, while the aperture helps to provide a clearer picture. Credit: Optica (2024). DOI: 10.1364/OPTICA.515726

"A soft X-ray provided pulse illumination at the speed of tens of femtoseconds (with one femtosecond being one-millionth of one-billionth of a second). The ultrashort duration of the radiation pulses enabled us to take an image before the structure of the living cell was altered by radiation damage," explained Egawa.

"We used Wolter mirrors for illumination and imaging. These mirrors provide a wide field of view, can withstand irradiation from the powerful lasers and exhibit no color distortion, making them ideal for observing samples at various wavelengths."

Although soft X-ray free electron lasers have previously been used to study smaller viruses and bacteria, mammalian cells were too big to be studied this way. However, by using Wolter mirrors, the team could achieve a wider field of view and use a thicker sample holder which could hold larger cells.

The resulting images showed details about carbon content in the cells that had not been seen through other methods, such as and fluorescence microscopy.

"It was surprising for us to find a carbon pathway between the nucleolus (a structure in the cell's nucleus, involved in cell function and survival) and the nuclear membrane (which envelops the nucleus), which had not been observed with visible light microscopes," said Egawa.

Brighter soft X-ray free electron lasers are available which would enable even clearer images with less grainy "noise." By adding brighter lasers and more precise Wolter mirrors, the team hopes to upgrade the microscope so that it can observe more biochemical elements. This could also help to illuminate some of the vital reactions and interactions which take place within living cells.

More information: Satoru Egawa et al, Observation of mammalian living cells with femtosecond single pulse illumination generated by a soft X-ray free electron laser, Optica (2024). DOI: 10.1364/OPTICA.515726

Journal information: Optica

Citation: Observing mammalian cells with superfast soft X-rays (2024, May 24) retrieved 25 June 2024 from https://phys.org/news/2024-05-mammalian-cells-superfast-soft-rays.html
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.

Explore further

New mirror that can be flexibly shaped improves X-ray microscopes

144 shares

Feedback to editors