This article has been reviewed according to Science X's editorial process and policies. Editors have highlighted the following attributes while ensuring the content's credibility:


peer-reviewed publication

trusted source

written by researcher(s)


Myrtle rust is devastating Australian forests: New high-tech spray holds out hope for native trees

Myrtle rust is devastating Australian forests: New high-tech spray holds out hope for native trees
One-to-two-year-old Syzygium jambos trees (n = 6) grown under glasshouse conditions were treated with nuclease-free H2O (negative control), a non-specific dsRNA control (green fluorescent protein (GFP)), or A. psidii-specific dsRNAs beta-tubulin (β-TUB), or transcription elongation factor (EF1-a) at 100 ng/μL and challenged with A. psidii urediniospore inocula 48 h post-dsRNA treatment. Disease was assessed two weeks post-inoculation. a Photo comparison of S. jambos trees, one from each treatment and control group. -dsRNA and GFP control trees showed severe symptoms of myrtle rust, whereas EF1-a and β-TUB-treated plants showed significantly reduced symptoms. Photos were taken two weeks post-inoculation. Credit: Communications Biology (2023). DOI: 10.1038/s42003-023-05618-z

Around a decade ago, an invasive fungal disease called myrtle rust reached Australia and began to spread like a plague through certain plants. The disease affects plants of the Myrtaceae family, which includes eucalypts, paperbarks and lilly pillies, and makes up 10% of Australian plant biodiversity.

In only a few years, myrtle rust has changed ecosystems by destroying trees and their canopies, wiped out whole species in certain areas, and taken an economic toll on industries that grow trees such as lemon-scented myrtle and tea tree.

The disease is a slow-moving ecological wrecking ball: surveys suggest it may drive at least 16 species of rainforest tree to extinction in the wild within a generation, with another 20 species at risk.

We have used RNA technology similar to that in COVID vaccines to create a highly targeted treatment for myrtle rust: a spray that can restore even severely infected trees to health in around six weeks. Our study was published in Communications Biology.

At-risk species in remote places

The current approaches to dealing with tree diseases are limited. We can apply fungicides with a scorched-earth policy to kill all fungi, or we can breed plants for resistance to the pathogen.

Neither of these strategies is effective against myrtle rust. There are too many species to defend, located in some of the most remote places imaginable.

For example, one tree species on the brink of extinction from myrtle rust is called Lenwebbia sp. Main Range. It grows only on cliff faces in the Nightcap Range in northern New South Wales.

What's more, many culturally significant and iconic trees in Australia and Aotearoa (New Zealand) are long-lived and cannot be swapped seasonally for resistant genotypes.

Myrtle rust is devastating Australian forests. A new high-tech spray holds out hope for native trees
Untreated (left) and treated (right) myrtle rust spores on artificial leaves (upper) and Syzygium jambos(rose apple) leaves (lower), 24 hours after infection. Untreated spores have germinated and produced infection structures that are used to penetrate into the leaf. Treated spores either do not germinate at all, or they are unable to produce the infection structures needed to.penetrate the leaf. Credit: Degnan et al, Communications Biology (2023). DOI: 10.1038/s42003-023-05618-z, CC BY

In the absence of a treatment for myrtle rust, the safeguard is to stockpile seeds to preserve genetics for future generations.

RNA interference

Our treatment for myrtle rust makes use of a molecular mechanism possessed by almost all plants, animals and fungi called "RNA interference."

RNA is an essential molecule of life, similar to DNA, which usually occurs in single strands. When a cell detects double-stranded RNA (which in nature generally represents a virus or other threat), it triggers RNA interference to destroy the interloper.

The RNA interference system learns to recognize the threat, and will then also destroy any single-stranded messenger RNA that happens to match. This naturally occurring mechanism can be used to defend both plants and humans against pathogens, including fungi.

We designed double-stranded RNA that matched in the fungus that causes myrtle rust, and sprayed it on the leaves of infected plants. This triggered the fungus's RNA interference mechanism, sabotaging the action of genes it needs to survive.

Treating severe infections

Rust fungi produce microscopic battering-ram structures called appressoria that are used to forcefully penetrate host leaves. Most treated with double-stranded RNA could not germinate to produce their battering rams, and those that did were withered and powerless.

Double-stranded RNA can also be sprayed onto plants ahead of time to inhibit spore germination and prevent disease completely.

Myrtle rust is devastating Australian forests. A new high-tech spray holds out hope for native trees
Untreated (left) and treated (right) Syzygium jambos (rose apple) trees six weeks after infection with myrtle rust. Two weeks after infection, treated plants were sprayed with double-stranded RNA targeting.myrtle rust. Six weeks after infection, the treatment has successfully inhibited myrtle rust and treated plants have re-grown and recovered to a healthy state. Credit: Degnan et al, Communications Biology (2023). DOI: 10.1038/s42003-023-05618-z, CC BY

Next we trialed whether our RNA spray would stop and cure disease in severely infected plants. We saw that it inhibited the progress of the disease, and after six weeks even severely infected plants had recovered to a healthy state.

A targeted treatment

We wanted to make sure our treatment wouldn't accidentally affect anything except the myrtle rust fungus, so we designed it using "barcoding genes" which uniquely identify the species.

Barcoding genes are excellent targets for RNA interference. They are generally identical among all members of a species, differ between closely related , and usually control an essential cellular function.

The most closely related rust fungus to the pathogen that causes myrtle rust is found on a naturalized street tree in Australia called Albizzia lebbeck, but it is different enough to be unaffected by our treatment. It is extremely unlikely any unrelated organism would have an identical barcoding gene sequence to the myrtle rust pathogen, so we do not expect any off-target effects.

Another advantage of targeting a barcoding gene is our treatment has lasting impact. Unlike some other genes, barcoding genes cannot change by mutation without risking the organism's survival.

This means the pathogen is less likely to evolve resistance. And if resistance against double-stranded RNA does evolve, the target sequence can be modified to match the rust again in a matter of days.

An integrated approach

There is no silver bullet to manage pathogens in native ecosystems and agriculture. The Australian government's myrtle rust action plan recommends an integrated approach to control this destructive disease.

In the coming years, double-stranded RNA can be incorporated to manage the epidemic of in Australia. We hope it will be especially useful in conservation, industry, and the treatment of individual trees—particularly culturally significant ones.

More information: Rebecca M. Degnan et al, Double-stranded RNA prevents and cures infection by rust fungi, Communications Biology (2023). DOI: 10.1038/s42003-023-05618-z

Journal information: Communications Biology

Provided by The Conversation

This article is republished from The Conversation under a Creative Commons license. Read the original article.The Conversation

Citation: Myrtle rust is devastating Australian forests: New high-tech spray holds out hope for native trees (2023, December 15) retrieved 19 July 2024 from
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.

Explore further

An environmentally friendly RNA-based spray to help combat rust disease in plants


Feedback to editors