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High-throughput mass spectrometry platform enables in-situ screening of fatty-acid-producing strains

High-throughput mass spectrometry platform enables in-situ screening of fatty-acid-producing strains
Graphical abstract. Credit: Talanta (2023). DOI: 10.1016/j.talanta.2023.125234

Mass spectrometry (MS) is a valuable analytical tool for high-throughput screening due to its speed, high resolution, and sensitivity. Recently, researchers from the Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT) of the Chinese Academy of Sciences (CAS) have developed a high-throughput MS platform that enables in-situ screening of fatty acid-producing strains.

The results were published in Talanta.

The proposed platform uses electrospray ionization (LAESI-MS) for direct profiling of triglyceride (TAG) species in cellular lipid droplets of Aurantiochytrium sp. The ionization source, one of the core components of the MS platform, integrates a high-energy laser module and an electrospray module.

The laser module serves to ablate colony cells and desorb molecules from cellular lipid droplets, generating molecular plumes, while the electrospray module ionizes the molecular plumes produced by laser ablation.

In the workflow of the platform, the laser source is precisely focused on a single colony from the agar plate. The laser energy ablates and desorbs the colony cells, resulting in the generation of a molecular plume. Subsequently, the plume is ionized by the electrospray, thereby converting the molecules into ions. Finally, the resulting ions are analyzed using a .

This MS platform enables rapid, highly effective sampling and ionization of TAG molecules. The distribution of fatty acids is estimated by TAG intensities in cellular lipid droplets of colonies on agar plates. Notably, it has realized a fast sampling rate of ≤ 2 s per sampling point and ≤ 13.69% relative standard deviation (RSDs) of the relative contents of fatty acids.

The MS platform has been applied to analyze Phaeodactylum tricornutum and Yarrowia lipolytica strains, demonstrating its capability for , which makes it an efficient tool for fatty acid biomanufacturing.

More information: Huan Liu et al, A high-throughput platform enables in situ screening of fatty acid-producing strains using laser ablation electrospray ionization mass spectrometry and a Python package, Talanta (2023). DOI: 10.1016/j.talanta.2023.125234

Citation: High-throughput mass spectrometry platform enables in-situ screening of fatty-acid-producing strains (2023, November 24) retrieved 3 March 2024 from https://phys.org/news/2023-11-high-throughput-mass-spectrometry-platform-enables.html
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