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Fluorescent protein sheds light on bee brains

Fluorescent protein sheds light on bee brains
The “sensor bee” can be used to investigate how bees process external stimuli and how their social behaviour is represented in the brain. Credit: Christian Verhoeven (

An international team of bee researchers involving Heinrich Heine University Düsseldorf (HHU) has integrated a calcium sensor into honey bees to enable the study of neural information processing including response to odors. This also provides insights into how social behavior is located in the brain, as the researchers now report in the scientific journal PLOS Biology.

Insects are important so-called model organisms for research. Despite more than 600 million years of independent evolution, insects share more than 60% of their DNA with humans. For several decades it was mainly the fruit fly whose could be used to study biological processes.

Later, such research was expanded to other insects, with particularly promising results coming from the . Bees display complex —they perform sophisticated behaviors while employing orientation, communication, learning and memory abilities, which make them interesting subjects for research into the brain's function and neural processing.

A team of researchers from the Universities in Düsseldorf, Frankfurt am Main, Paris-Saclay and Trento has now developed a method to enable direct observation of bee brains, a work which has now been published in PLOS Biology.

A was integrated into the neurons. Calcium plays an important role in nerve cell activity. "We modified the genetic code of honey bees to make their brain cells produce a fluorescent protein, a sort of sensor that allows us to monitor the areas that are activated in response to environmental stimuli. The intensity of the light emitted varies according to ," explains Dr. Albrecht Haase, Professor of Neurophysics at the University of Trento.

Professor Beye indicates that "the realization of this 'sensor bee' was particularly challenging because we had to work on the DNA of queen bees. Unlike , the queen bee cannot easily be maintained in the laboratory, because each one needs its own colony."

The research started with the inoculation of a specific genetic sequence into over 4,000 bee eggs. The protracted breeding, testing and selection process ultimately resulted in seven queens carrying the genetic sensor. When they reproduced in their own colony, the queens transmitted the gene to some of their offspring.

The sensor developed by the team of researchers was then used to study the bees' sense of smell and how the perception of smell is encoded in the neurons. Dr. Julie Carcaud, Assistant Professor at the University of Paris-Saclay and Dr. Jean-Christophe Sandoz, Research Director at CNRS in Paris, explain, "The insects were stimulated with various odors and observed with a high-resolution microscope. This made it possible to detect which brain cells are activated by these smells and how this information is distributed in the ."

Dr. Marianne Otte, co-author of the study from Düsseldorf, says, "The recordings were performed in vivo using techniques which enabled us to look into the brains of the bees. The insects were fixed in a measuring stand and then presented with various odor stimuli."

Professor Dr. Bernd Grünewald, from Goethe University Frankfurt am Main and Director of the Honeybee Research Center in Oberursel, says, "The new 'sensor bee' makes it possible to study how communication works within colonies and, more generally, how sociality affects the animals' brains."

More information: Julie Carcaud et al, Multisite imaging of neural activity using a genetically encoded calcium sensor in the honey bee, PLOS Biology (2023). DOI: 10.1371/journal.pbio.3001984

Journal information: PLoS Biology

Provided by Heinrich-Heine-Universität Düsseldorf

Citation: Fluorescent protein sheds light on bee brains (2023, March 3) retrieved 28 May 2023 from
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