Changing carbohydrates into lipids for microalgae biofuels

Microalgae biofuels: Changing carbohydrates into lipids
Figure 1: Electron microscope image of lipid production in the microalgae Chlamydomonas sp. Credit: Kobe University

A cross-institutional collaboration has developed a technique to repartition carbon resources from carbohydrates to lipids in microalgae. It is hoped that this method can be applied to biofuel production. This discovery was the result of a collaboration between a research group at Kobe University's Engineering Biology Research Center consisting of Project Assistant Professor Kato Yuichi and Professor Hasunuma Tomohisa et al., and Senior Researcher Satoh Katsuya et al. at the Takasaki Advanced Radiation Research Institute of the Quantum Beam Science Research Directorate (National institutes for Quantum and Radiological Science and Technology).

These research results were published in the international academic journal Communications Biology.

Biofuels are that have received much attention in the move towards creating more sustainable societies. Microalgae are that are highly capable of producing lipids from in the atmosphere, making them promising candidates for . However, a Kobe University research group consisting of Project Assistant Professor Kato Yuichi and Professor Hasunuma Tomohisa et al. discovered that the majority of carbon resources were diverted to starch production instead of production under light/dark conditions (i.e. day and night). This is a problem when cultivating species outside.

Research Methodology

For this research study, Project Assistant Professor Kato and Professor Hasunuma's Kobe University research group collaborated with Senior Researcher Satoh et al. at the National institutes for Quantum and Radiological Science and Technology (QST). The researchers used the ion beam at QST's Takasaki Advanced Radiation Research Institute to induce mutation in the microalgae. This enabled them to cultivate a new mutant strain called Chlamydomonas sp. KOR1, which can produce large quantities of lipids even in light/dark conditions.

Microalgae biofuels: Changing carbohydrates into lipids
Figure 2: Lipid production model created by disrupting the starch debranching enzyme in microalgae.

The researchers discovered that this KOR1 strain has disruptions in the starch debranching enzyme gene ISA1, causing it to produce a different carbohydrate: phytoglycogen instead of starch (Figure 1).

Normally, microalgae synthesize and accumulate carbohydrates (starch) during light periods and break them down when it is dark. However, many carbohydrates accumulate that cannot be completely broken down. Contrary to this, the carbohydrate synthesized by KOR1 (phytoglycogen) was completely broken down during the dark period. The results of the KOR1 metabolome analysis revealed a total increase in intermediate metabolites in both the starch and lipid synthesis pathways (intermediate metabolites included fructose-6-phosphate, glucose-6-phosphate, acetyl-CoA and glycerol 3-phosphate). From this analysis, the researchers illuminated the metabolic mechanism underlying the increased lipid production that resulted from ISA1 gene disruption. In the KOR1 strain, the carbohydrate (phytoglycogen) was quickly broken down and intermediate metabolites subsequently induced the carbon resource to be repartitioned to lipid production (Figure 2).

In order to produce biofuels using microalgae, it is necessary to cultivate these organisms outside in the sunlight. However, there is an unavoidable decrease in lipid production under these light/dark conditions. The technique of 'repartitioning carbon resources by disrupting the starch debranching enzyme gene' developed through this research is one answer to this problem. It is hoped that this new method can contribute towards the large-scale implementation of biofuel production using microalgae.


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More information: Yuichi Kato et al. Enhancing carbohydrate repartitioning into lipid and carotenoid by disruption of microalgae starch debranching enzyme, Communications Biology (2021). DOI: 10.1038/s42003-021-01976-8
Journal information: Communications Biology

Provided by Kobe University
Citation: Changing carbohydrates into lipids for microalgae biofuels (2021, May 6) retrieved 23 June 2021 from https://phys.org/news/2021-05-carbohydrates-lipids-microalgae-biofuels.html
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