A remote control for neurons

A remote control for neurons
Graphene flakes are grown on silicon nanowires to achieve superior conductivity. Credit: Carnegie Mellon University College of Engineering

A team led by researchers at Carnegie Mellon University has created a new technology that enhances scientists' ability to communicate with neural cells using light. Tzahi Cohen-Karni, associate professor of biomedical engineering and materials science and engineering, led a team that synthesized three-dimensional fuzzy graphene on a nanowire template to create a superior material for photothermally stimulating cells. NW-templated three-dimensional (3-D) fuzzy graphene (NT-3DFG) enables remote optical stimulation without need for genetic modification and uses orders of magnitude less energy than available materials, preventing cellular stress.

Graphene is abundant, cheap, and biocompatible. Cohen-Karni's lab has been working with graphene for several years, developing a technique of synthesizing the material in 3-D topologies that he's labeled "fuzzy" graphene. By growing two-dimensional (2-D) flakes out-of-plane on a silicon nanowire structure, they're able to create a 3-D structure with broadband optical absorption and unparalleled photothermal efficiency.

These properties make it ideal for cellular electrophysiology modulation using light through the optocapacitive effect. The optocapacitive effect alters the cell membrane capacitance due to rapidly applied light pulses. NT-3DFG can be readily made in suspension, allowing the study of cell signaling within and between both 2-D cell systems and 3-D, like human cell-based organoids.

Systems like these are not only crucial to understanding how cells signal and interact with each other, but also hold great potential for the development of new, therapeutic interventions. Exploration into these opportunities, however, has been limited by the risk of cellular stress that existing optical remote-control technologies present. The use of NT-3DFG eliminates this risk by using significantly less energy, on a scale of 1-2 orders of magnitude less. Its biocompatible surface is easy to modify chemically, making it versatile for use with different cell types and environments. Using NT-3DFG, photothermal stimulation treatments could be developed for motor recruitment to induce muscle activation or could direct tissue development in an organoid system.

A remote control for neurons
Nanowires are able to stimulate neurons from outside the cell membrane. Credit: Carnegie Mellon University College of Engineering

"This is an outstanding collaborative work of experts from multiple fields, including neuroscience through Pitt and UChicago, and photonics and through UNC and CMU," said Cohen-Karni. "The developed technology will allow us to interact with either engineered tissues or with nerve or muscle tissue in vivo. This will allow us to control and affect tissue functionality using light remotely with high precision and low needed energies."

Additional contributions to the project were made by Maysam Chamanzar, assistant professor of electrical and computer engineering. His team's core expertise in photonics and neurotechnologies assisted in developing the much-needed tools to allow both the characterization of the unique hybrid-nanomaterials, and in stimulating the cells while optically recording their activity.

A remote control for neurons
Neurons respond to optical stimulus from NT-3DFG nanostructures. Credit: Carnegie Mellon University College of Engineering

"The broadband absorption of these 3-D nanomaterials enabled us to use light at wavelengths that can penetrate deep into the tissue to remotely excite nerve cells. This method can be used in a whole gamut of applications, from designing non-invasive therapeutics to basic scientific studies," said Chamanzar.

The team's findings are significant both for our understanding of cell interactions and the development of therapies that harness the potential of the human body's own . Nanostructures created using NT-3DFG may have a major impact on the future of human biology and medicine.


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More information: Sahil K. Rastogi el al., "Remote nongenetic optical modulation of neuronal activity using fuzzy graphene," PNAS (2020). www.pnas.org/cgi/doi/10.1073/pnas.1919921117
Citation: A remote control for neurons (2020, June 1) retrieved 6 July 2020 from https://phys.org/news/2020-06-remote-neurons.html
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