Team develops rapid authentication method of Chinese medicines

January 10, 2018, Hong Kong Polytechnic University
PolyU's research team led by Dr Yao Zhongping, Associate Professor of the Department of Applied Biology and Chemical Technology, has developed a new method for rapid authentication of Chinese herbal medicines, including Lingzhi, and Tianma. Credit: The Hong Kong Polytechnic University

The Food Safety and Technology Research Centre under the Department of Applied Biology and Chemical Technology of The Hong Kong Polytechnic University (PolyU) has developed a new method for rapid authentication of Chinese herbal medicines, including Ganoderma (known as Lingzhi in Chinese), and Gastrodiae Rhizoma (known as Tianma in Chinese). The new method is quick and simple, taking around 10 minutes to analyse one raw sample, achieving authentication of genuine and counterfeit species, classification of wild types and cultivated types, as well as differentiation of geographical origins. This method can be further applied to other herbal medicines.

Lingzhi and Tianma are two of the most popular and valuable Chinese medicines. Due to their high commercial values, counterfeiting, adulterating and conflating Lingzhi and Tianma species is common in the market. Conventionally, fingerprint chromatography is adopted to authenticate and differentiate the species, as it can provide comprehensive chemical composition of a sample. However, it is a labor-intensive and time-consuming method, requiring several hours for sample preparation and the separation process for one sample.

Recently, the PolyU research team used direct ionization mass spectrometry (DI-MS) to detect the major active components of Lingzhi (ganoderic acids) and Tianma (gastrodin, parishin B/parishin C/and parishin). By directly applying a high voltage on a raw sample loaded with solvents, spray ionization could be induced at the tip of the to generate corresponding mass spectra within minutes. The presence of major active components in the DI-MS spectra authenticates genuine samples from counterfeit ones. Moreover, with Principal Component Analysis (PCA), a commonly used dataset analytic tool, wild and cultivated types can be classified, while could be differentiated.

Team develops rapid authentication method of Chinese medicines
Credit: Hong Kong Polytechnic University

There are approximately 80 Lingzhi species. Only two of them, Chizhi and Zizhi, are described in Chinese Pharmacopeia. Some other Lingzhi species with similar appearances are commonly confused with the official species. On the other hand, Tianma is easily confused with two counterfeit , namely Cacalia davidii (Franch.) Hand.-Mazz. and Canna edulis Ker.

Both wild Lingzhi and Tianma are very rare, and farm cultivation has become the primary source. In general, wild types have higher contents of major active components and thus better curative effects. Samples from different geographical locations also have variations in components due to varying cultivation conditions.

The method developed by PolyU is simple, rapid, reproducible and can be easily adopted by researchers in relevant fields as no additional specialized device is required. It has the potential to be further expanded for analysis of other , for example, Heshouwu and Wuweizi, and is therefore expected to have a positive impact on the Chinese herbal industry.

The research findings were published in Analytica Chimica Acta, a leading journal in analytical science.

Explore further: Team develops new method for rapid authentication of edible oils and screening of gutter oils

More information: Wong, H. Y. et al. Rapid Differentiation of Ganoderma Species by Direct Ionization Mass Spectrometry, Anal. Chim. Acta 2018, 999, 99-106.

Wong, H. Y. et al. Rapid Authentication of Gastrodiae Rhizoma by Direct Ionization Mass Spectrometry, Anal. Chim. Acta 2016, 938, 90-97.

Related Stories

Sniffing out fake perfumes

September 25, 2013

Scientists at EPFL have designed a quick method for detecting counterfeit perfumes, and have tested it on major brands like Givenchy, Hermes and D&G.

New technique for rapid detection of drugs-of-abuse

March 24, 2015

The development of reliable, convenient and cost-effective methods for drug analysis is crucial in the control and the fight against drug abuse. Hong Kong Polytechnic University (PolyU) has developed a new technique, named ...

Recommended for you

A new polymer raises the bar for lithium-sulfur batteries

January 18, 2018

Lithium-sulfur batteries are promising candidates for replacing common lithium-ion batteries in electric vehicles since they are cheaper, weigh less, and can store nearly double the energy for the same mass. However, lithium-sulfur ...

Looking to the sun to create hydrogen fuel

January 18, 2018

When Lawrence Livermore scientist Tadashi Ogitsu leased a hydrogen fuel-cell car in 2017, he knew that his daily commute would change forever. There are no greenhouse gases that come out of the tailpipe, just a bit of water ...

The early bits of life

January 18, 2018

How can life originate before DNA and genes? One possibility is that there are natural processes that lead to the organisation of simple physical objects such as small microcapsules that undergo rudimentary forms of interaction, ...


Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.