Environmental chemist flashes warning light on new nanoparticle

August 30, 2017, University of Massachusetts Amherst
A schematic diagram illustrating mechanisms of BP toxicity, which include disruption of cell membrane integrity related to layered BP particle size and generation of reactive oxygen species. Baoshan Xing at UMass Amherst, with colleagues in China, report the findings in a cover story in Small. Credit: UMass Amherst/Baoshan Xing

When environmental and soil chemist Baoshan Xing at the University of Massachusetts Amherst began reading in 2014 that a new, two-dimensional material known as layered black phosphorous (BP) was gaining the attention of biomedical researchers for use in drug delivery systems and tumor photothermal therapy, he was both intrigued and concerned.

"I am not only a soil chemist, but an environmental chemist," he notes. "As agricultural scientists, we are very familiar with phosphorous but I had never heard of two-dimensional black phosphorous. So we read all the nice papers about black phosphorous, and then, as environmental chemists, we started asking about nanoparticle toxicity. You have to be careful where you put such materials in the human body."

In a recent cover story of the journal, Small, his former postdoctoral fellow, Qing Zhao, currently a professor at the Institute of Applied Ecology at the Chinese Academy of Sciences, and Xing report toxicity test results for different thicknesses of layered BP in three cell lines. Briefly, they found disruption of cell related to layered BP particle size, plus concentration- and cell-type-dependent cytotoxicity.

Xing says, "We are among the first ones to work with this material, particularly in regard to its environmental implications." He and colleagues urge that "an in-depth understanding of BP's cytotoxicity is of utmost importance" to provide useful data for risk evaluation and safe biomedical applications.

The researchers acknowledge that the new material, which is collected as thin samples from phosphorous crystals by a technique known as exfoliating, that is, shaving off layers of different thicknesses, does have "unique optical and electrical properties," which might make it "a promising candidate for an efficient vehicle and photothermal/photodynamic therapy in treating a variety of cancers."

Xing says, "I remember when single-layer graphene generated great excitement in the research community a decade ago, and I think people are getting excited now about a single layer of black phosphorous, that it might have many exciting applications." But the two materials differ a great deal in their single-layer structure, he adds, where single-layer graphene is perfectly flat, exfoliated BP has a zig-zagged structure.

Zhao, Xing and their colleagues point out that studies of layered BP toxicity conducted to date have used viability reagents, which can interfere with cytotoxicity results. By contrast, they have used a label-free, real-time cell analysis (RTCA) technique that does not need any fluorescent or colorimetric viability reagents.

Assaying layered BP toxicity in three cell types, mouse fibroblast (NIH 3T3), human colonic epithelial cells (HCoEpiC) and human embryonic kidney cells (293T), the UMass Amherst and Chinese research team found that layered BP's cytotoxicity is based on the fact that it generates reactive oxygen species (ROS). ROS are among the most potent cell-damaging agents known. Layered BP also disrupts cell membrane integrity in a particle-size-dependent manner. "The larger the BP is, less membrane integrity will be retained," they note.

Further, they say the IC50 values of layered BP can differ by dozens of times depending on particle size and cell type. IC50 values refer to a measure of how effective a material is in inhibiting a specific biological function. Xing and colleagues urge that "special attention should be paid to the size of layered BP and the types of target cell lines for its application in biomedical field."

They add, "Further study is undoubtedly necessary to explore the cytotoxicity mechanisms in depth," and that "given the results from our present study, the mechanisms of BP's are strikingly complicated and have significant implications for the risk evaluation and safe biomedical applications of BP."

They plan to follow up with further experiments to test their hypothesis that layered BP, with its unusual electrical properties, might prove useful in removing both positively and negatively charged chemicals and organic contaminants from water.

Explore further: Phosphorene reveals its superconductivity

More information: Xuejiao Zhang et al. Size Effect on the Cytotoxicity of Layered Black Phosphorus and Underlying Mechanisms, Small (2017). DOI: 10.1002/smll.201701210

Related Stories

Keeping cool with a black semiconductor

June 13, 2016

As anyone who has held a laptop computer or cell phone knows, they produce heat that has the potential to damage the microchips inside. However, layered, crystalline black phosphorus could lead to a new microchip design that ...

Black phosphorus doesn't mind de-aerated water

December 1, 2016

Researchers at the Center for Multidimensional Carbon Materials (CMCM), within the Institute for Basic Science(IBS) have discovered that one of graphene's competitors, black phosphorus, is inert to water deprived of oxygen, ...

Travelling through the body with graphene

September 28, 2016

For the first time researchers succeeded to place a layer of graphene on top of a stable fatty lipid monolayer. Surrounded by a protective shell of lipids graphene could enter the body and function as a versatile sensor. ...

Recommended for you

Scientists have a new way to gauge the growth of nanowires

March 19, 2018

In a new study, researchers from the U.S. Department of Energy's (DOE) Argonne and Brookhaven National Laboratories observed the formation of two kinds of defects in individual nanowires, which are smaller in diameter than ...

Rubbery carbon aerogels greatly expand applications

March 19, 2018

Researchers have designed carbon aerogels that can be reversibly stretched to more than three times their original length, displaying elasticity similar to that of a rubber band. By adding reversible stretchability to aerogels' ...

Plasmons triggered in nanotube quantum wells

March 16, 2018

A novel quantum effect observed in a carbon nanotube film could lead to the development of unique lasers and other optoelectronic devices, according to scientists at Rice University and Tokyo Metropolitan University.

Zero field switching (ZFS) effect in a nanomagnetic device

March 16, 2018

An unexpected phenomenon known as zero field switching (ZFS) could lead to smaller, lower-power memory and computing devices than presently possible. The image shows a layering of platinum (Pt), tungsten (W), and a cobalt-iron-boron ...

Imaging technique pulls plasmon data together

March 16, 2018

Rice University scientists have developed a novel technique to view a field of plasmonic nanoparticles simultaneously to learn how their differences change their reactivity.


Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.