Protein team produces molecular barrels

Aug 05, 2013
Protein team produces molecular barrels
The protein machineries TOM und SAM are linked via Tom22 and work together in the maturation process of beta-barrel structures of proteins. Credit: Becker et al., 2008; Biochim. Biophys. Acta 1777, 447-563 Thomas Becker (BBA-2008)

Research groups headed by Prof. Dr. Nikolaus Pfanner, Dr. Nils Wiedemann, and Dr. Thomas Becker from the University of Freiburg and their colleagues have demonstrated how molecular protein barrels form in the outer membrane of the mitochondria, the powerhouses of the cell. Their studies revealed that two protein machineries cooperate in an unexpected way. The researchers published their findings in the scientific journal Cell.

Mitochondria are essential for the survival of the cell, rendering such vital services as providing the energy for . Mitochondria are surrounded by two membranes. The outer membrane contains characteristic proteins with a barrel-like structure, the beta-barrel structure. These proteins extend across the membrane and are crucial for the transport of proteins and into the mitochondria. The proteins are produced as precursors in the cytosol, only forming their mature barrel structure upon entering the . They are imported through the pores of the protein complex TOM, the translocase of the outer mitochondrial membrane, and then transported to a second in the , the sorting and assembly machinery SAM. Finally, the SAM complex integrates the proteins into the membrane. The individual steps leading to the formation of the beta-barrel structure and the transfer of the from TOM to SAM were not previously understood.

The researchers studied the formation of the beta-barrel structure within the context of a partnership between the Collaborative Research Center 746 "Functional Specificity by Coupling and Modification of Proteins," the Cluster of Excellence BIOSS Centre for Biological Signalling Studies, and the Spemann Graduate School of Biology and Medicine.

The team headed by Nils Wiedemann demonstrated that the beta-barrel structure forms at the SAM complex. The PhD student Jian Qiu discovered that the receptor protein Tom22 plays a key role in this process. This comes as a surprise, because it was previously thought that TOM and SAM were independent protein machineries. However, findings from Thomas Becker's research group showed that the two complexes directly interact with each other. They are linked by Tom22, as the PhD student Lena-Sophie Wenz discovered. If Tom22 is not present, the molecular bridge between TOM and SAM is lost – severely hampering the formation of beta-barrel structures. The findings of this study demonstrate that the direct transfer of the imported protein from the TOM complex to the SAM complex enables an efficient formation of mitochondrial beta-barrel structures.

Explore further: Top Japan lab dismisses ground-breaking stem cell study

More information: Qiu, J. et al. (2013) Coupling of Mitochondrial Import and Export Translocases by Receptor-Mediated Supercomplex Formation. Cell, Volume 154, Issue 3, 596-608. DOI: 10.1016/j.cell.2013.06.033

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