Mapping a route to stem cell therapies

May 20, 2013
Mapping a route to stem cell therapies
Dr Jose Polo has mapped the stem cell re-programming process for the first time. Credit: ThinkStock

Monash University researchers are shedding light on the complex processes that underpin the creation and differentiation of stem cells, bringing closer the promise of 'miracle' therapies.

Dr Jose Polo of the Australian Institute (ARMI) and the Department of Anatomy and and his team, with collaborators at Harvard, have comprehensively mapped, for the first time, the process by which are re-programmed to become an induced pluripotent stem (iPS) cell.
iPS cells behave almost exactly like - they can become any cell in the body - but come without the ethical and scientific pitfalls.

The re-programming process was developed in 2006; however, until now, it was unknown exactly how it worked.

Dr Polo has unravelled the precise molecular events occurring in an adult at almost every level throughout the re-programming process. The results were published in the prestigious journal Cell.

Every cell in the body contains a full genome or complete set of DNA. The differences between a brain neuron and a are down to the fact that although they contain the exact same genes, not all of these are active in each cell - a process known as transcription.

Proteins called control which genes are active or inactive by changing the availability of the genes to be transcribed. This in turn mediates the differentiation of stem cells to all the different types of cells in the body. It is the reversal of this complex process - returning an to its pluripotent state - that the researchers mapped.

"Once you understand a process, you can manipulate it," Dr Polo said.

"Now that we have created a roadmap for re-programming, we can do a lot of things. We can look at improving the efficiency of re-programming and investigating if some of the potential dangers can be avoided by re-routing the process."

Now, Dr Polo's team is investigating whether re-programming follows an identical path for all types of cells. They are also looking at the potentially cancer-causing mutations in iPS cells and ways to circumvent this tumorific potential.

Another project is examining "memory" in iPS cells and its implications.

"For example, if I create an iPS cell from a blood cell and an iPS cell from a muscle cell, the two are a little different - they carry memories of where they were generated. Now I'm examining ways to exploit these memories," Dr Polo said.

Dr Polo said these and many more complexities must be understood before stem cell therapy can move effectively into the clinical arena.

"Modern medicine has achieved wonderful things, but doctors are like mechanics trying to fix a car without access to spare parts," Dr Polo said.

"The promise of stem cells is amazing - it will almost bring us into a new age of medicine - but there's a lot more work to do before this promise can be realised."

Explore further: Deadly bacterium uses cellular proteins as bait to sabotage a pathway that enables immune response

Related Stories

Novel probe for live human iPS cell imaging

May 16, 2013

Researchers from the National Institute of Advanced Industrial Science and Technology (AIST) have developed a highly sensitive lectin probe, rBC2LCN, for human induced pluripotent stem cells (iPS cells). ...

Recommended for you

The vital question: Why is life the way it is?

6 hours ago

The Vital Question: Why is life the way it is? is a new book by Nick Lane that is due out on April 23rd. His question is not one for a static answer but rather one for a series of ever sharper explanations—explanations that a ...

Food poisoning: New detection method for bacterial toxin

7 hours ago

The Bacillus cereus bacteria is one of the potential causes of food poisoning. Indeed, a recent study in Analytical and Bioanalytical Chemistry shows that this versatile pathogen produces 19 different varian ...

Detailing heterochromatin formation at the onset of life

7 hours ago

Antoine Peters and his group at the Friedrich Miescher Institute for Biomedical Research (FMI) have elucidated the mechanisms controlling the packaging of chromatin in the early embryo. They have identified ...

User comments : 0

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.