Cheap and quick HIV testing made possible with DVD scanners

Apr 11, 2013
Cheap and quick HIV testing made possible with DVD scanners
The Lab on DVD unit.

Thanks to USB sticks and video streaming, DVD players are becoming all but obsolete. But their cheap optics may find a new life in a cost-effective and speedy technique for on-the-spot HIV testing and other analytics.

Aman Russom, senior lecturer at the School of Biotechnology at KTH Royal Institute of Technology in Stockholm, says that his research team converted a commercial DVD drive into a microscope that can analyse blood and perform with one-micrometre resolution. The breakthrough creates the possibility of an inexpensive and simple-to-use tool that could have far-reaching benefits in health care in the developing world.

"With an ordinary DVD player, we have created a cheap analytical tool for DNA, RNA, proteins and even entire cells," says Russom. The so-called "Lab-on-DVD" technology makes it possible to complete an in just a few minutes, he says.

In a proof of , the researchers collected cell-type CD4 + from blood and visualized it using the DVD reader technology. Enumeration of these cells using is now standard in HIV testing, but the practice has been limited in developing countries.

The Lab-on-DVD reaps 30 years of research and development on optical storage technology to create an alternative to flow cytometry, the standard equipment for hospitals. Flow cytometry units can cost upwards of USD 30,000, excluding maintenance. By contrast, mass-produced Lab-on-DVD units could be made available for less than USD 200, Russom says. And unlike the bulky and technically-complex flow cytometry instruments, a Lab-on-DVD would be portable and require less training to operate.

"The low cost of the technology makes it suitable as a diagnostic and analytical tool in clinical practice close to the patient," Russom says. "And because it delivers extremely fast analysis, the patient does not need to go home and wait for a response. They can get it right on the first visit to a doctor."

The researchers' work was highlighted by Nature Photonics in April: www.nature.com/nphoton/journal… nphoton.2013.64.html

Explore further: UCI team is first to capture motion of single molecule in real time

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