This article has been reviewed according to Science X's editorial process and policies. Editors have highlighted the following attributes while ensuring the content's credibility:

fact-checked

peer-reviewed publication

trusted source

proofread

Researchers discover how nature repurposes ammonium transporters as receptors

How nature repurposes ammonium transporters as receptors
Membrane protein Sd-Amt1 in the bacterium Shewanella denitrificans. Credit: Susana Andrade

A team led by Freiburg biochemist Prof. Dr. Susana Andrade has characterized a new membrane protein that allows microorganisms to repurpose ammonium transporters (Amts) as receptors.

Ammonium transporters clearly distinguish between , potassium and water. This property has certainly contributed to their conversion into ammonium in the course of evolution.

The newly discovered protein Sd-Amt1 utilizes ammonium cations as extracellular signals to increase the cytoplasmic level of the secondary messenger cyclic-di-GMP. It consists of a membrane-integral ammonium receptor domain linked to a cytoplasmic diguanylate cyclase transducer module.

The first authors of the study are Freiburg biochemists Dr. Tobias Pflüger and Dr. Mathias Gschell from the University of Freiburg. The results have been published in the journal Science Advances.

The research team's work combines modern biochemical, biophysical and bioinformatics tools to integrate high-resolution structural and functional data. They enabled the researchers to reveal the molecular details of ammonium-triggered signal binding, receptor activation and transducer modulation.

In collaboration with the bioinformatics group of Prof. Dr. Stefan Günther from the Institute of Pharmaceutical Bioinformatics at the University of Freiburg, they were also able to show the extent to which the signal binding motif is conserved between prokaryotic and . The work thus also provides a signature motif for identifying other ammonium receptors.

More information: Tobias Pflüger et al, How sensor Amt-like proteins integrate ammonium signals, Science Advances (2024). DOI: 10.1126/sciadv.adm9441

Journal information: Science Advances

Citation: Researchers discover how nature repurposes ammonium transporters as receptors (2024, June 6) retrieved 24 June 2024 from https://phys.org/news/2024-06-nature-repurposes-ammonium-receptors.html
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.

Explore further

Biochemists show how evolution combines a nutrient sensor from existing elements

102 shares

Feedback to editors