This article has been reviewed according to Science X's editorial process and policies. Editors have highlighted the following attributes while ensuring the content's credibility:

fact-checked

peer-reviewed publication

trusted source

proofread

Research team develops biotechnological process to degrade plastics

Research team develops biotechnological process to degrade plastics
pH effect on the CALB:BHET reactant complex conformations. a Detail of the structure of CALB with the computed pKa values for uncertain titratable residues. Positions Cα of key residues in the protein are shown as spheres. b Computed titration curves for key amino acid residues of CALB generated by constant-pH neMD/MC simulations. c Detail of the interactions between Asp134 and Gln157 and Ser105 created in the active site in the CALB: BHET reactant complex at pH 5 and 9. d Population analysis of geometrical parameters (Nu···Csp2=O Bürgi-Dunitz angle in degrees, and Nu···Csp2 distance in Å) defining the nucleophilic attack of Ser105 to C1 atom of BHET. e Population analysis defining the relative position of Hγ of Ser105 and its activator His224, and of Hγ of Ser105 and Asp134, at pH 5 and 9. Credit: Nature Communications (2023). DOI: 10.1038/s41467-023-39201-1

The lack of knowledge about the molecular mechanisms that make biocatalysis possible is an obstacle to developing biotechnological processes that allow the recycling of plastics. A research project led by a team from the Universitat Jaume I has made it possible to degrade widely used PET plastics through a natural enzyme, CALB, by modifying the pH of the medium. This opens up a new way to recycle PET, which is present, for example, in containers, bottles or textiles of all kinds, and generates harmless compounds that are useful in subsequent synthesis processes.

The results were published in the journal Nature Communications by a computational biochemistry team from the Institute of Advanced Materials (INAM) of the UJI led by Vicent Moliner and Katarzyna Świderek, in collaboration with a group from the Center for Cooperative Research in Biomaterials (CIC biomaGUNE) of the Basque Country, led by Fernando López Gallego, and another from the University of the Basque Country, led by Haritz Sardon. These last two groups carried out the experimental part of the project.

Moliner explains, "As did not exist historically, evolution has not had time to develop enzymes capable of degrading these materials." Plastics have very interesting properties, leading to their extensive use in different applications. However, it is precisely one of these properties (their durability) that gives rise to the problem of recycling them.

"Researchers are wondering how to recycle them efficiently and without generating environmental problems. In this regard, one of the objectives of our group at the INAM is to design enzymes that could degrade plastics. As computational chemists, we made the predictions based on theoretical simulations and our colleagues from the Basque Country carried out the experimental demonstration," says Moliner.

Research team develops biotechnological process to degrade plastics
Vicent Moliner and Katarzyna Świderek of the Institute of Advanced Materials of the UJI. Credit: Asociacion RUVID

The study was based on a (CALB) which, according to Moliner, "unlike most enzymes, is capable of catalyzing more than one reaction; it is very promiscuous. This is how we discovered that not only can we use this to degrade PET, but by modifying the pH of the medium, the structure of the enzyme varied and we were able to reach two types of products that are difficult to obtain by traditional methods of synthesis, and which are useful to obtain others."

The INAM researcher stresses that in this way "we have discovered a very simple mechanism that allows us to degrade a in and at low temperature, in addition to controlling the end products of the reaction by simply modulating the pH."

The next step, Moliner indicates, is "to continue delving deeper into the study in order to improve the speed of the reaction by redesigning the enzyme." Another line they are currently exploring is to advance in the degradation of other plastics such as polyurethanes, another type of synthetic polymer widely used today—a point on which the INAM Computational Biochemistry group is working with other research groups in Sweden and Denmark.

More information: Katarzyna Świderek et al, Mechanistic studies of a lipase unveil effect of pH on hydrolysis products of small PET modules, Nature Communications (2023). DOI: 10.1038/s41467-023-39201-1

Journal information: Nature Communications

Provided by Asociacion RUVID

Citation: Research team develops biotechnological process to degrade plastics (2023, November 6) retrieved 24 February 2024 from https://phys.org/news/2023-11-team-biotechnological-degrade-plastics.html
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.

Explore further

Study lays foundations for future medicine design

26 shares

Feedback to editors