Study identifies a new mechanism involved in the reproductive function

Study identifies a new mechanism involved in the reproductive function
Molecular mechanisms underlying Kiss1 neuronal alterations in KiDKO mice. Relative expression of Kiss1 promoter repressors, Mkrn3, Sirt1, Eap1, Cux1, and members of the Polycomb group, Eed, Cbx7, and Yy1, were analyzed in ARC Kiss1 neurons isolated by FACS from control and KiDKO male and female mice (a) and in AVPV Kiss1 neurons from control and KiDKO female mice (c). In addition, Kiss1 promoter activators, Ttf1 and Mll1, were measured in the same ARC (b) and AVPV (d) Kiss1 neuronal samples. Groups sizes: n = 5 control females; n = 7 KiDKO females; n = 5 control males; n = 4 KiDKO males. The values are represented as the mean ± SEM. *P < 0.05 vs. corresponding control groups. Credit: Nature Communications (2022). DOI: 10.1038/s41467-022-32347-4

A family of regulatory molecules, microRNAs, are key to the maintenance of fertility in both genders.

An international research team led by the Maimonides Biomedical Research Institute(IMBIC) and the UCO has found that the brain, through regulatory molecules called microRNAs—whose function is to control which genes of a cell are expressed—is able to influence the reproductive capacity of both males and females.

The study, published in the journal Nature Communications, confirms that microRNAs affect "the and the maintenance of reproduction in the adult stage," according to Manuel Tena-Sempere, a researcher with the "Hormonal regulation of energy balance, puberty and reproduction" group at the IMIBIC and a professor in the UCO's Cell Biology, Physiology and Immunology Department, and co-director, together with Juan Roa, of the study.

Specifically, the team studied the impact of microRNAs on Kiss1 neurons, which are essential for both the pubertal activation of the reproductive axis and for this reproductive axis to function well throughout adulthood. In fact, alterations affecting the Kiss1 system have been associated with the absence of puberty and infertility in humans.

Using in which the synthesis of microRNAs was suppressed in Kiss1 neurons only, the research team found that the mice developed long-term infertility; that is, microRNAs in Kiss1 neurons are indispensable to the reproductive function because these molecules are responsible for influencing repressors in Kiss1 neurons. Without the microRNAs, these repressors prevent the proper functioning of the reproductive function.

The study has also shown that the time course of microRNA deletion differs between the sexes, in line with differences in the complexity of their reproductive mechanisms, which is generally greater in females. Without microRNAs in Kiss1 neurons, male mice initiated puberty and could become fertile for a short time, while females never reached .

Although this is a basic study whose implications will not be immediate, its results open up new avenues of research, from understanding the basis of some pathologies that affect reproduction, such as infertility, to the use of microRNAs in therapeutic treatments.

More information: Juan Roa et al, Dicer ablation in Kiss1 neurons impairs puberty and fertility preferentially in female mice, Nature Communications (2022). DOI: 10.1038/s41467-022-32347-4

Journal information: Nature Communications

Citation: Study identifies a new mechanism involved in the reproductive function (2022, September 28) retrieved 30 November 2022 from
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