How Streptococcus pyogenes can survive on skin and cause skin infections

How Streptococcus pyogenes can survive on skin and cause skin infections
Figure. Streptococcus pyogenes on the skin up-regulates the ADI pathway, leading to bacterial survival and disease. WT indicates wild-type S. pyogenes (blue cells) that up-regulate ADI under low-glucose conditions, such as the skin surface. When ADI is activated, the bacteria can survive using arginine instead of glucose: virulent bacteria penetrate and damage the skin. ΔADI indicates S. pyogenes that has been genetically modified to inactivate the ADI pathway (red cells), leading to low virulence. In blood, ADI is suppressed by high glucose levels. Credit: Osaka University

Streptococcus pyogenes is one of the most important bacterial causes of human skin infections. If S. pyogenes invades deep into the tissue, it can cause life-threatening illnesses, such as sepsis and toxic shock. With its limited supply of carbohydrates, the skin is generally an effective barrier against infection and not a good surface for the survival of S. pyogenes. To survive successfully and invade deep into the tissue, bacteria must be able to find a source of nutrients and also evade the skin's immune defenses.

Now, an international team led by Osaka University, Japan, in collaboration with Keio University, Japan, and University of California San Diego, U.S., has discovered a way this disease organism obtains nutrition from the surface. This knowledge could lead to new therapeutic approaches to tackle infections. The team recently published the work in Cell Reports.

It was already known that some bacteria break down arginine (an amino acid—one of the building blocks of proteins) via a named the arginine deaminase (ADI) pathway. The team confirmed that S. pyogenes can survive using arginine even when starved of glucose. When they deleted the (named arcA) encoding the first enzyme in the ADI pathway, the S. pyogenes lost that ability and also became less toxic towards human skin cells grown in culture.

When arginine is supplied, the ADI pathway of S. pyogenes is activated but there are also widespread changes in . Some genes are down-regulated but large numbers of genes are up-regulated, including genes associated with virulence of S. pyogenes, such as those that produce bacterial toxins.

Using mouse skin as a model system for human skin, the team showed for the first time that S. pyogenes can use arginine to survive on the skin surface. S. pyogenes lacking the arcA gene survived poorly and were less virulent on mouse skin, confirming the importance of this pathway in the disease process. In contrast, in blood (containing plentiful glucose), the altered S. pyogenes appeared no different to normal S. pyogenes since the ADI pathway was not needed.

"We showed that arginine from stratum corneum-derived filaggrin was a key substrate for the ADI pathway of S. pyogenes," says lead researcher Dr. Yujiro Hirose. "In mice that do not produce filaggrin, less arginine is available and the S. pyogenes bacteria do not utilize ADI pathway to cause the skin lesion."

"This represents a significant step forward in understanding how S. pyogenes survives on the skin," explains Dr. Hirose. "We expect our study will lead to novel treatment strategies: if we can block arginine metabolism in S. pyogenes it should restrict the pathogen's survival and virulence."

More information: Yujiro Hirose et al. Streptococcus pyogenes upregulates arginine catabolism to exert its pathogenesis on the skin surface, Cell Reports (2021). DOI: 10.1016/j.celrep.2021.108924

Journal information: Cell Reports

Provided by Osaka University

Citation: How Streptococcus pyogenes can survive on skin and cause skin infections (2021, March 31) retrieved 30 November 2023 from
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.

Explore further

Cytotoxins contribute to virulence of deadly epidemic bacterial infections


Feedback to editors