Fungi complex active ingredients could have potential for medical applications

Impulse for Research on Fungi
In expansion microscopy, the preparation is magnified more than four times. Here, a germ tube of Aspergillus fumigatus is shown before and after expansion; the scale corresponds to ten micrometers. The plasma membrane (turquoise) and the mitochondria (pink) were stained. Credit: Ulrich Terpitz / University of Würzburg

Fungi play an important role for mankind. In the soil, they decompose dead organic material, making it accessible to plants as a nutrient. In industrial biotechnological plants, fungi produce vast quantities of chemicals and food every day. In addition, fungi produce very complex active ingredients that could have potential for medical applications.

On the other hand, there are fungi that damage crops or make people sick. For example, infections caused by the fungus Aspergillus fumigatus can be fatal—especially people with a severely weakened , for example after stem cell or organ transplantation, are affected.

Despite this great importance, far less is known about fungi than about other organisms. "New findings in also require that as many researchers as possible have access to state-of-the-art ," says Dr. Ulrich Terpitz from the Biocenter at Julius-Maximilians-Universität (JMU) Würzburg in Bavaria, Germany.

Successful with expansion microscopy

Terpitz's team has developed such a method in collaboration with three other research groups: For the first time, fungi can now be visualised using expansion microscopy (ExM). This method makes visible with a resolution of less than 60 nanometres—using a conventional confocal fluorescence microscope.

"Although this is less than can be achieved with high-tech super-resolution fluorescence microscopes, access to such facilities is often limited, especially for fungal researchers. In contrast, standard fluorescence microscopes are widely used and expansion microscopy can be carried out in any biological laboratory," said Terpitz.

The JMU working groups of Professor Markus Sauer (Biocenter) and Dr. Johannes Wagener (Institute of Hygiene and Microbiology) and the group "Molecular Genetics of Fungal Pathogenicity" headed by Professor Antonio Di Pietro from the University of Cordoba in Spain contributed to the development.

Method used for three fungal species

The ExM procedure is quite simple: the stained fungus sample is embedded in a polyacrylamide hydrogel and the fluorescent dyes are anchored in the hydrogel. After water is added, the polymer expands like a gummy bear in a glass of water. It also expands the fluorescent dyes in a uniform manner.

However, there is a problem to be solved first: Fungi have a cell wall that must be removed before expansion. The researchers use cell wall dissolving enzymes for this purpose. "The result is an easy-to-handle protocol for the ExM of that can be used for different fungus species, including the clinically relevant Aspergillus fumigatus," said Terpitz. The researchers also applied their method to the pathogens of corn blight (Ustilago) and tomato wilt (Fusarium oxysporum).


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More information: Ralph Götz et al. Expansion Microscopy for Cell Biology Analysis in Fungi, Frontiers in Microbiology (2020). DOI: 10.3389/fmicb.2020.00574
Citation: Fungi complex active ingredients could have potential for medical applications (2020, April 8) retrieved 6 July 2020 from https://phys.org/news/2020-04-fungi-complex-ingredients-potential-medical.html
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