Speedy and precise multicolor imaging of biomolecules now possible

Speedy and precise multicolor imaging of biomolecules now possible
The use of gold, silver and gold-silver alloy nanoparticles allowed high-speed/high-precision multicolor imaging for the first time. Credit: Ryota Iino, Institute for Molecular Science, National Institutes of Natural Sciences, Japan.

For the first time, researchers can track biological molecules with unprecedented speed and precision thanks to the use of multi-metallic nanoparticles.

The researchers published their results on October 17 in ACS Photonics, a journal of the American Chemical Society.

Nanoparticles are used to track the movements of isolated from cells and also in living cells, such as the mechanisms related to intracelluar transport, , and other processes. Researchers have traditionally used to track these movements, but, in imaging, they could only show one color: green. Now, scientists can see more than green through the use of gold, silver and gold-silver alloy nanoparticles.

"Gold nanoparticles are very powerful tools used to precisely track the fast motion of biomolecules," said Ryota Iino, paper author and professor at the Institute for Molecular Science in the National Institutes of Natural Sciences. "However, the imaging was previously limited to monochromatic green. In this study, by using gold, silver and silver-gold nanoparticles, we have succeeded in extending the color palette—between purple and green—of high-speed and high-precision imaging of biomolecules."

Other tagging techniques, such as organic fluorescent dyes, can extend the color palette to include reds, but they tend to display as weaker colors than the sharp and strong showing that give off. Metallic nanoparticles are also more stable than organic dyes, meaning they remain visible as they move with the tagged biomolecule for a long period.

"Nanoparticles show much stronger signals, and they don't blink in the same way organic dyes can," Iino said. "Different nanoparticles also strongly scatter the light at different wavelengths, meaning they show up as visibly different colors when imaged."

The team is now working on extending the imaging even further with newly engineered nanoparticles. They also hope to use even much smaller to have a better understanding of all molecular mechanisms in functioning cells.

More information: Jun Ando et al, Multicolor High-Speed Tracking of Single Biomolecules with Silver, Gold, and Silver–Gold Alloy Nanoparticles, ACS Photonics (2019). DOI: 10.1021/acsphotonics.9b00953

Journal information: ACS Photonics

Provided by National Institutes of Natural Sciences

Citation: Speedy and precise multicolor imaging of biomolecules now possible (2019, December 9) retrieved 16 July 2024 from https://phys.org/news/2019-12-speedy-precise-multicolor-imaging-biomolecules.html
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