Gold-DNA nanosunflowers for efficient gene silencing and controlled transformation

Gold-DNA nanosunflowers for efficient gene silencing and controlled transformation
Scheme of self-assembled gold-DNA nanosunflowers for enhanced cellular uptake amount, tunable gene silencing efficacy, and controlled tumor inhibition effect by NIR irradiation. (A) (a) Assembly and disassembly of the large-sized nanostructure (200-nm gold-DNA nanosunflowers) from/to ultrasmall nanoparticles (2-nm Au-POY2T NPs). (b) Representative TEM image of the nanosunflowers. (c) Masterpiece: Sunflowers (Vincent van Gogh, 1889). (B) Left: In vivo tumor retention and penetration of transformable nanosunflowers. Right: Enhanced cellular uptake and controlled oncogene silencing process of the nanosunflowers in vitro. ① Large-sized nanosunflowers were taken up by an MCF-7 cell. ② The nanosunflowers standby in the cell cytoplasm. ③ Upon NIR irradiation, large-sized gold-DNA nanostructures dissociate and release small units (2-nm Au-POY2T NPs) to attack the cell nucleus. ④ The silencing sequence POY2T will bind to the P2 promoter of the c-myc oncogene and down-regulate the c-myc expression of MCF-7 cells, which can be controlled (ON/OFF) and regulated (Low/Medium/High) by the NIR irradiation. Credit: Science Advances, doi: 10.1126/sciadv.aaw6264

Developing an efficient delivery system for enhanced and controlled gene interference-based therapeutics is an existing challenge in molecular biology. The advancing field of nanotechnology can provide an effective, cross-disciplinary strategy to facilitate nucleic acid delivery. In a new report, Shuaidong Huo and colleagues in the interdisciplinary departments of Nanoscience, Interactive Materials, Chemistry and Polymer Research in China, Germany and the U.S. used triplex-forming oligonucleotide sequences coupled to its complementary strand to mediate the self-assembly of ultra-small gold nanoparticles.

The resulting sunflower-like nanostructures showed strong near infrared (NIR) absorption and ability for photothermal conversion. When the scientists irradiated the structures with NIR, the larger nanostructures disassembled to generate ultra-small nanoparticles modified with the c-Myc oncogene sequence to directly target the cancer cell nucleus. Huo et al. controlled gene silencing by synergistically controlling the time of preincubating cells with nanoparticles alongside nanostructure self-assembly (in vitro and in vivo) and the time-frame of NIR irradiation. The study provided a new paradigm to construct efficient and tailored nanocarriers for applications of gene interference and therapeutic gene delivery.

Gene therapy has great potential to treat a variety of diseases and complications including infertility, HIV and cancer. Successful gene therapy to alleviate disease symptoms depend on an efficient gene delivery vehicle or vector. During the process, the gene carrier must cross many biological barriers and cell membranes while escaping endosomal entrapment and nuclease-based degradation. Compared to virus-based delivery strategies, non-viral gene delivery approaches face many challenges during the process of loading and releasing DNA/RNA, targeted delivery and intracellular uptake, including incompatibility relative to immune responses in vivo.

Vigorous efforts in nanotechnology are underway to engineer stable and efficient vehicles for gene transfer to cancer cells. Due to their unique physiochemical properties a number of nanomaterials have emerged for gene delivery. Among them, gold nanoparticles (Au NPs) with specific size and surface properties can overcome obstacles in vivo to become one of the most studied gene carrier systems. However, these strategies have encountered a variety of shortcomings and therefore it is important to establish efficient delivery systems or enhanced and controlled gene therapies.

Self-assembly and testing sunflower-like nanostructures

In the present work, Huo et al. were inspired by nature's ability to hybridize DNA by engineering DNA-mediated, self-assembled gold DNA nanostructures (approximating 200 nm). The sunflower-like design showed strong NIR absorption and photothermal conversion properties. Upon NIR irradiation, the structures disassembled to liberate ultra-small gold nanoparticles (2 nm, Au NPs) with potential for oncogene silencing, improved cell and nuclei permeability and enhanced transfection efficiency. The scientists synergistically controlled the cell-nanomaterial interactions based on the time of pre-incubation in the lab, followed by time of circulation in vivo and the timeline of irradiation. The experiments facilitated increased cellular uptake, tunable gene silencing efficacy and controlled tumor inhibition. The transformable nanosunflowers provided an excellent model to design nanovehicles for drug delivery with great potential in biomedicine.

Gold-DNA nanosunflowers for efficient gene silencing and controlled transformation
Morphology characterization of the self-assembled nanostructures (nanosunflowers). (A) TEM (200 kV) images of the nanosunflowers with enlarged structural details. (B) Bio-TEM (80 kV) images with enlarged polymer structural details. (C) High-resolution TEM (200 kV) images showing the distribution of ultrasmall NPs on the self-assembled nanostructure. (D) SEM images with enlarged surface topography of the nanosunflowers. Credit: Science Advances, doi: 10.1126/sciadv.aaw6264

Huo et al. first synthesized the two-nanometer Au NPs coated with tiopronin and modified them with thiol-oligonucleotides (SH-POY2T) using an established method of ligand exchange. The 23-nucleotide (nt) POY2T oligonucleotide bound the P2 promoter of the c-myc oncogene to form a triplex structure and downregulate oncogenic c-myc expression. In parallel, they designed and synthesized another single-stranded sequence known as CA to complementarily hybridize to the tail of the POY2T sequence and block its binding to the c-myc oncogene. On completion, the nanostructure self-assembled into sunflower-like structures. The team investigated the nanostructure (200 nm) using transmission electron microscopy (TEM). Additional imaging revealed further details of the DNA moieties of the "sunflower" structure. When the materials scientists used scanning electron microscopy (SEM) to validate the TEM results, they observed consistency between the methods.

They investigated the UV-Vis absorption spectra of the ultrasmall Au NPs prior to DNA-mediated . The monodispersed, individual two-nanometer Au-POY2T NPs showed strong absorption in the NIR region to generate heat under NIR irradiation. Huo et al. credited the observed strong NIR absorbance to close interparticle spacing and nonuniform spatial distribution of individual NPs within the larger nanostructure. They tested the heat response of the self-assembled nanostructures under NIR irradiation and noted the melting point of the complementary DNA sequences (POY2T and CA) to approximate 41 degrees C, dissociating half of the duplex structure between complementary DNA sequences. Huo et al. selected 10 minutes as the optimal time for NIR irradiation in the study.

Gold-DNA nanosunflowers for efficient gene silencing and controlled transformation
Photothermal property and disassembly behavior study of the self-assembled nanostructures. (A) Visible absorption spectra of 2-nm core-sized NPs and 200-nm self-assembled nanostructures. a.u., absorbance unit. (B) Temperature response of self-assembled nanostructures, upon NIR irradiation, dispersed in water and cell culture medium. Mean values ± SD, n = 3. (C) Temperature rise of self-assembled nanostructures, upon NIR irradiation, dispersed in water and cell culture medium. (D) Change of maximum absorbance (767 nm) of 2-nm core-sized NPs and 200-nm self-assembled nanostructures upon NIR irradiation. (E and F) TEM observation of disassembly behavior of 200-nm self-assembled nanostructures before (top) and after (bottom) NIR irradiation (808 nm, 10 min). (G) Hydrodynamic diameter of (a) monodispersed 2-nm Au-POY2T NPs and size change of the 200-nm nanosunflowers before (b) and after (c and d) NIR irradiation for different time periods (3 and 10 min). Credit: Science Advances, doi: 10.1126/sciadv.aaw6264

Disassembly behaviour of the self-assembled nanostructures and proof-of-concept

The scientists hypothesized the self-assembled nanostructures would shrink and disassemble into individual ultrasmall Au-POY2T NPs. After 10 minutes of NIR irradiation, the maximum absorption (767 nm) of nanostructures markedly decreased to disassemble the sunflower structure. They followed the experiments before and after NIR irradiation with TEM observations and used particle size analysers to understand the disassembly process and size transformation of the nanostructures up to six nanometers in size and confirmed the optimal suitability of the 10-minute timeline.

Huo et al. applied NIR irradiation to MCF-7 cells treated with self-assembled gold DNA nanostructures and tested their cellular uptake in vitro as proof-of-concept. They determined the cellular internalization of Au-POY2T (2 nm) across diverse incubation times and quantified their cellular uptake using inductively coupled plasma mass spectroscopy (ICP-MS) and previous methods. They noted increased internalization after six hours of incubation compared to 24-hour incubation timelines. They did not observe inhibitors of endocytosis to influence Au-POY2T NP uptake, suggesting the involvement of an alternative path such as membrane fusion.

Understanding gene silencing behavior of the self-assembled nanostructures

Gold-DNA nanosunflowers for efficient gene silencing and controlled transformation
Controlled nucleus localization and gene silencing study in vitro of the self-assembled nanostructures. (A) Schematic of the in vitro cell experimental setup for the controlled NP nucleus localization and gene regulation study. (B) Number of 2-nm Au-POY2T NPs localized in the MCF-7 cell nucleus with treatment of ① individual 2-nm Au-POY2T NPs, ② 200-nm nanosunflowers, and 200-nm nanosunflowers with NIR irradiation (10 min) after different preincubation times (③ 1, ④ 3, ⑤ 6, and ⑥ 12 hours). Mean values ± SD, n = 3. Statistical differences were determined by two-tailed Student’s t test; *P < 0.05 and **P < 0.01. (C) Confocal observation of distribution of fluorescein isothiocyanate–labeled nanosunflowers (green) before (top) and after (bottom) NIR irradiation in MCF-7 cells. Nucleus was labeled by 4′,6-diamidino-2-phenylindole (blue). (D) Bio-TEM image of the localization of large-sized nanosunflowers (top, red arrow) in the cytoplasm and distribution of released small NPs (bottom, blue arrow) in cytoplasm and nucleus after NIR irradiation in MCF-7 cells. (E) Cytotoxicity evaluation of MCF-7 cells with treatment of 200-nm nanosunflowers after NIR irradiation (after a period of preincubation time: 1, 3, 6, and 12 hours, respectively) compared to control, 2-nm Au-TIOP NPs, POY2T sequence, CA sequence, 2-nm Au-POY2T NPs, 200-nm nanosunflowers without NIR irradiation, and NIR exposure only. All the concentrations of treatments were at or equal to 1 μM in POY2T sequence and were tested after a total of 24 hours of incubation. Mean values ± SD, n = 3. Statistical differences were compared with the treatment group of ① individual 2-nm Au-POY2T NPs determined by two-tailed Student’s t test; *P < 0.05 and **P < 0.01. (F) C-myc mRNA level determined by real-time PCR after different treatments as described above. Mean values ± SD, n = 3. Statistical differences were determined by two-tailed Student’s t test; **P < 0.01 and ***P < 0.001. (G) C-myc protein levels determined by Western blot and (H) corresponding quantitative histogram after different treatments as described above. GAPDH, glyceraldehyde phosphate dehydrogenase. Credit: Science Advances, doi: 10.1126/sciadv.aaw6264

After enhanced cellular uptake of self-assembled nanostructures in vitro, the research team investigated the distribution of nanoparticles within the cell nuclei using "standby" and "attack" strategies after NIR triggering. For this, they extracted cell nuclei after incubation, for ICP-MS analysis after NIR irradiation across diverse periods of incubation (one, three, six and 12 hours). They noted that the pre-incubation period largely affects nanoparticle internalization within the cell nucleus, and the researchers regulated Au-POY2T NPs in the cell nucleus based on the time of pre-incubation and NIR irradiation.

Huo et al. also investigated NIR-irradiation controlled therapeutic effects of nanosunflowers using cell viability tests; they observed oncogene silencing to increase markedly (80 percent) and kill more cancer cells. The research team controlled the therapeutic impact effectively by changing the timeline of pre-incubation and irradiation efficiently. The results supported a superior ability of the transformable nanosunflowers to silence the c-myc oncogene and oncoprotein. The scientists controlled the gene silencing process by tuning pre-incubation timelines prior to NIR irradiation.

Controlling tumor growth inhibition using self-assembled nanosunflowers

To test the controllable anti-tumor efficiency of nanosunflowers in vivo, the scientists first investigated their blood compatibility to confirm good blood biocompatibility. The research team then established the MCF-7 tumor model using the BALB/c nude mice, allowed the tumor volumes to reach 50 mm3 and randomly divided the animals into nine groups and treated them with 1000 µl of varying POY2T formulations. After each injection, they irradiated the animal groups with NIR lasers for 10 minutes to reach a local temperature above 41 degrees C.

Gold-DNA nanosunflowers for efficient gene silencing and controlled transformation
Controlled tumor growth inhibition study of the self-assembled nanostructures. (A) The MCF-7 tumor BALB/c nude mice model was established at day 0. After tumors were ready, the mice were randomly divided into nine groups and treated with 100 μl of various formulations (equivalent to 10 μM in POY2T sequence; group ① with 2-nm Au-POY2T NPs and groups ②, ③, ④, ⑤, and ⑥ with 200-nm nanosunflowers) at days 9, 12, and 15. In groups ③, ④, ⑤, and ⑥, the tumors were irradiated with a NIR laser for 10 min at 1, 3, 6, and 12 hours after each intravenous injection. Saline, NIR only, and POY2T were used as control groups. The (B) body weights and (C) tumor volumes were measured every 3 days. Scale bar, 1 cm. After the mice were sacrificed at day 24, all tumors were (D) isolated and (E) weighted, respectively. Mean values ± SD, n = 4. Statistical differences were determined by two-tailed Student’s t test; *P < 0.05, **P < 0.01, and ***P < 0.001. (Photo credit: Ningqiang Gong, National Center for Nanoscience and Technology, China.) (F) Hematoxylin and eosin staining images of organs including the heart, liver, spleen, lung, kidney, and tumor after different treatments. Scale bar, 200 μm. Credit: Science Advances, doi: 10.1126/sciadv.aaw6264

Of note, mice treated with the nanosunflower-treated group and irradiated at 12 hours showed the most significant anti-tumor effects, indicating efficient delivery of gene silencing units into the tumor site. After 24 days, Huo et al. sacrificed the animals, isolated the tumors and weighed them to demonstrate nanosunflower based NIR-controlled tumor growth inhibition in vivo. Based on histological studies, the team showed the treatment significantly reduced tumor growth and did not affect the morphology of other organs. The results verified the therapeutic efficiency and lack of side effects for nanosunflowers and NIR therapy.

In this way, Shuaidong Huo and colleagues designed, developed and optimized nanoagents for effective anti-tumor therapy. They engineered self-assembled sunflower-like nanostructures to act as multiparticle carriers loaded with many ultrasmall therapeutic units. Upon NIR irradiation, the nanostructures dissociated to release swarms of small NPs to target the cell nucleus. In tumor-bearing mice, the large sunflowers passively targeted the tumor site followed by NIR irradiation to transform the tumor genetic composition and shrink it. The research team aim to improve transfection efficiency and provide a blueprint for controllable gene silencing at tumor sites using transformable gene interference carriers for intricate theranostics at the level of the single cell.

More information: Shuaidong Huo et al. Gold-DNA nanosunflowers for efficient gene silencing with controllable transformation, Science Advances (2019). DOI: 10.1126/sciadv.aaw6264

Reinhard Waehler et al. Engineering targeted viral vectors for gene therapy, Nature Reviews Genetics (2007). DOI: 10.1038/nrg2141

N. L. Rosi. Oligonucleotide-Modified Gold Nanoparticles for Intracellular Gene Regulation, Science (2006). DOI: 10.1126/science.1125559

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Citation: Gold-DNA nanosunflowers for efficient gene silencing and controlled transformation (2019, October 28) retrieved 20 April 2024 from
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