New technologies enable better-than-ever details on genetically modified plants

New technologies enable better-than-ever details on genetically modified plants
This image depicts an Arabidopsis plant overlaid on individual, labeled DNA molecules of the T-DNA-transformed Arabidopsis genome. Credit: Salk Institute

Salk researchers have mapped the genomes and epigenomes of genetically modified plant lines with the highest resolution ever to reveal exactly what happens at a molecular level when a piece of foreign DNA is inserted. Their findings, published in the journal PLOS Genetics on January 15, 2019, elucidate the routine methods used to modify plants, and offer new ways to more effectively minimize potential off-target effects.

"This was really a starting point for showing that it's possible to use the latest mapping and sequencing technologies to look at the impact of inserting into the ," says Howard Hughes Medical Institute Investigator Joseph Ecker, a professor in Salk's Plant Molecular and Cellular Biology Laboratory and head of the Genomic Analysis Laboratory.

When a scientist wants to put a new gene into a plant—for basic research purposes or to boost the health or nutrition of a food crop—they usually rely on Agrobacterium tumefaciens to get the job done. Agrobacterium is the bacteria that causes crown gall tumors, large bulges on the trunks of trees. Decades ago, scientists discovered that when the bacteria infected a tree, it transferred some of its DNA to the tree's genome. Since then, researchers have co-opted this transfer ability of Agrobacterium for their own purposes, using its transfer-DNA (T-DNA) to move a desired gene into a plant.

Recently, DNA sequencing technologies had started to hint that when the Agrobacterium T-DNA is used to insert new genes into a plant, it may cause additional changes to the structural and chemical properties of the native DNA.

"Biotech companies spend a lot of time and effort to characterize transgenic plants and disregard candidates with unwanted changes without understanding—from a basic biological perspective—why these changes may have occurred," says Ecker. "Our new approach offers a way to better understand these effects and may help to speed up the process."

"The biggest unknown was whether, and how many copies of, the T-DNA were inserted at the same time as the piece you wanted," says Florian Jupe, a former Salk research associate who now works at Bayer Crop Science. Jupe, Salk Staff Researcher Mark Zander and Research Assistant Angeline Rivkin are co-first authors of the new paper, along with Todd Michael of the J. Craig Venter Institute.

Since the T-DNA approach can lead to an integration of many copies of a desired gene into a plant, it can be difficult to study the final result with standard DNA sequencing, as most technologies struggle to sequence highly repetitive stretches of DNA. But Ecker and his colleagues turned to a new combination of approaches—including optical mapping and —to look at these long stretches in high resolution. They applied the technologies to four randomly selected T-DNA lines of Arabidopsis thaliana, a commonly used model plant in biology. (These plants are derived from a large population of T-DNA insertional mutants that were created using an Arabidopsis transformation method, called floral dip, to study gene function.)

Optical mapping revealed that the plants had between one and seven distinct insertions or rearrangements in their genomes, ranging in size by almost tenfold. Nanopore sequencing and reconstruction of the genomes of two lines confirmed the insertions to single-letter resolution, including whole segments of DNA that had been exchanged—or translocated—between chromosomes in one of the selected lines. Gene insertions themselves showed a variety of patterns, with the inserted DNA fragment sometimes scrambled, inverted or even silenced.

"This study was not possible even a year ago," says Michael. "Nanopore sequencing, which some refer to as the 'holy grail' of DNA sequencing, has revolutionized the reading of even the most complex regions of the genome that were completely inaccessible and unknown until now."

Finally, when the researchers studied packets of genetic material called histones they found additional changes. Histone proteins package DNA into structural units, and modifications of these histones mediate whether a gene can be accessed for use by a cell (a level of regulation called epigenetics). Depending on where T-DNA was integrated, certain nearby histone modifications appeared or disappeared potentially changing the regulation or activation of other nearby genes.

"Now we have the first high-resolution insights on how T-DNA insertions can shape the local epigenome environment," says Zander.

In an ideal world, the researchers say, T-DNA would insert one single, functional copy of a desired gene with no nearby side effects on a plant's genome. While their findings show this is rarely the case in Arabidopsis, their methods offer a path to a better understanding and surveillance of the effects.

"This technology is exciting because it gives us a much clearer look at what's going on in some of these transgenic Arabidopsis lines," says Rivkin.

"With Arabidopsis, it's relatively easy because it has such a small genome, but because of continued improvements in DNA sequencing technology, we expect this approach will also be possible for crop ," adds Ecker, who holds the Salk International Council Chair in Genetics. "Current methods require screening of hundreds of transgenic lines to find good performing ones, such as those without extra insertions, so this technology could provide a more efficient approach."


Explore further

Extensive variation revealed in 1,001 genomes and epigenomes of Arabidopsis

More information: Florian Jupe et al, The complex architecture and epigenomic impact of plant T-DNA insertions, PLOS Genetics (2019). DOI: 10.1371/journal.pgen.1007819
Journal information: PLoS Genetics

Provided by Salk Institute
Citation: New technologies enable better-than-ever details on genetically modified plants (2019, January 19) retrieved 18 June 2019 from https://phys.org/news/2019-01-technologies-enable-better-than-ever-genetically.html
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Jan 19, 2019
Not only in Plants, but also in Animals ! We have so much potential in Genetics on our hands. Take 20 Breeds of Dogs. Do their whole Genomic sequencing. Then, mate them together and find whole genominc sequencing of their progeny. FIND WHAT HAPPENED and use that knowledge.
The Earlier the Research, The Better !

Jan 20, 2019
When "discussing" genetically engineered plants, "science" always "assures" that it is necessarily safe. They depict the process in a simple fashion, just putting a gene in between two other genes.
This article indicates that it is an uncontrolled by product of infection of the host plant with a bacterium. No mention is made of what other effects the bacterium may have, other than inserting genes into the host. They article says that there can be "desired" and "undesired" outcomes of infecting the plant with a gene. The article describes alterations in histones, which wasn't wished. And uses the term "in an ideal world:, indicating that what really happens in genetic engineering is far from what would be wished.

Jan 21, 2019
And uses the term "in an ideal world:, indicating that what really happens in genetic engineering is far from what would be wished.


And the article also said:
". . . their methods offer a path to a better understanding and surveillance of the effects.
This technology is exciting because it gives us a much clearer look at what's going on in some of these transgenic Arabidopsis lines," says Rivkin.


The article is about learning more to overcome the negative effects of editing. This is how progress is made- incremental steps.


Jan 24, 2019
Not only in Plants, but also in Animals ! We have so much potential in Genetics on our hands. Take 20 Breeds of Dogs. Do their whole Genomic sequencing. Then, mate them together and find whole genominc sequencing of their progeny. FIND WHAT HAPPENED and use that knowledge.
The Earlier the Research, The Better !

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