Scientists from the University of Basel have succeeded in organizing spherical compartments into clusters mimicking the way natural organelles would create complex structures. They managed to connect the synthetic compartments by creating bridges made of DNA between them. This represents an important step towards the realization of so-called molecular factories. The journal Nano Letters has published their results.
Within a cell there are specialized compartments called organelles, as for example nucleus, mitochondria, peroxisomes and vacuoles that are responsible for specific functions of the cell. Almost all sophisticated biological functions of cells are realized by self-organization, a process by which molecules adopt a defined arrangement based on their specific conformations and properties, without outside guidance.
Using self-organization of nano-objects into complex architectures is a major strategy to produce new materials with improved properties or functionalities in fields such as chemistry, electronics and technology. For example, this strategy has already been applied to create networks of inorganic solid nanoparticles. However, so far, these networks were not able to mimic sophisticated structures that have biological functions within the cells and thus have potential application in medicine or biology.
DNA bridges give stability
The joint research of the groups led by the professors Cornelia Palivan and Wolfgang Meier now provides a new approach to self-organize artificial organelles into clusters that mimics the connection between their natural counterparts. By using single DNA strands to interconnect the spherical compartments the scientists succeeded in creating clusters according to a specific architecture and controlled properties. "We were excited to see, that the different DNA strands on the surface of the spherical compartment migrated together and formed a bridge with the DNA strands from the next one", says Palivan. This DNA bridge represents an extremely stable connection.
This strategy inspired by nature goes beyond the actual self-organization approaches, since it also allows the integration of various requirements such as the fine tuning of the distance between the compartments or different topologies "on demand". As compartments, the scientists used polymersomes, with a synthetic membrane that, unlike liposomes, has the great advantage of being very stable and controlling the fusing of individual compartments within the cell.
An additional unique advantage of this strategy to organize nano-clusters is the fact that the compartments can be loaded with reaction partners such as enzymes, proteins or catalysts. This gives the basis for the further engineering of artificial organelles serving as molecular factories. This research was done within the National Centre of Competence in Research (NCCR) Molecular Systems Engineering.
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Juan Liu, Viktoriia Postupalenko, Samuel Lörcher, Dalin Wu, Mohamed Chami, Wolfgang Meier, Cornelia G. Palivan: DNA-mediated self-organization of polymeric nanocompartments leads to interconnected artificial organelles. Nano Letters (2016), DOI: 10.1021/nanolett.6b03430