Team demonstrates protein damage by shock waves in traumatic brain injury patients

January 27, 2016, Lawrence Livermore National Laboratory
When a body is exposed to a blast, the collapse of tiny bubbles can magnify the damage on brain cell membranes.

New research by Lawrence Livermore scientists shows how shock waves can damage membrane proteins in traumatic brain injury patients.

Blast-induced (TBI) from improvised explosive devices is the most frequent wound occurring from the conflicts in Afghanistan and Iraq. Estimates suggest more than 200,000 veterans have had at least one traumatic brain injury.

Clinical reports and in vivo studies show exposure to a blast can cause TBI, although how the energy is transmitted to the brain is not well understood.

That's where Livermore researchers come in. Using molecular dynamics (MD) simulations, LLNL physicists Ed Lau and Eric Schwegler, along with University of North Carolina (link is external) colleague Max Berkowitz, found that are resistant to damage by shock waves. But with the presence of bubbles, the damage from shock waves is magnified and can contribute to an electrolyte imbalance within cells that can lead to the initial symptoms of TBI, such as headaches and seizures.

When a body is exposed to a blast, shock waves are produced, resulting in shear forces within the cranium. How cells react to shock waves is not well understood.

Cell walls are composed mainly of lipids but contain a large number of proteins that are vital for the normal function of the cell. The team performed all-atom MD simulations on shock-wave-induced bubble collapse to determine its impact on a nerve membrane-bound protein.

Previous experiments and molecular simulations of bubble-shock interactions have shown that the force generated by bubble collapse is great enough to cause pores to form in membranes, known as poration. These pores likely lead to unregulated ion exchange through the membrane that can lead to a cascade of events that cause injury and ultimately neuronal death.

"Ionic imbalances likely play an important role in the cellular damage incurred from TBI," Schwegler said.

Ion channel dysfunction in humans is known to be a factor in irregular heartbeats and in diseases such as cystic fibrosis and epilepsy. Molecular simulations have shown that membranes can self-heal from nanometer-size pores in tens of nanoseconds. In this study, the team demonstrated that alone do not significantly damage membrane-bound proteins (ion channel). But shock-wave-induced bubble collapse can have adverse effects on membrane-bound ion channels even at velocities that are too slow to affect the membrane.

"The damage to the organism from faulty ion regulation can be severe, since, unlike a membrane, ion channels may not self-heal as rapidly," Lau said.

The research appears in the Jan. 5 edition of Biophysical Journal.

Explore further: A single mild blast exposure can cause brain injuries with similarities to Alzheimer's disease

More information: Edmond Y. Lau et al, Shock Wave-Induced Damage of a Protein by Void Collapse, Biophysical Journal (2016). DOI: 10.1016/j.bpj.2015.11.030

Related Stories

Studying dynamics of ion channels

May 18, 2015

Scientists from the Vaziri lab at the Vienna Biocenter, together with colleagues at the Institute for Biophysical Dynamics at the University of Chicago, have developed a method using infrared spectroscopy and atomistic modeling ...

When touch turns to pain

October 13, 2015

Researchers in Tübingen and Trieste (Italy) have made a major contribution to understanding the sense of touch and pain. A team led by Dr Jing Hu (Werner Reichardt Centre for Integrative Neuroscience – CIN, Tübingen) ...

Recommended for you

Field-responsive mechanical metamaterials (FRMMs)

December 11, 2018

In a recent study published in Science Advances, materials scientists Julie A. Jackson and colleagues presented a new class of materials architecture called field-responsive mechanical metamaterials (FRMM). The FRMMs exhibit ...

Researchers develop smartphone-based ovulation test

December 11, 2018

Investigators from Brigham and Women's Hospital are developing an automated, low-cost tool to predict a woman's ovulation and aid in family planning. Capitalizing on advancements in several areas, including microfluidics, ...

CRISPR method for conditional gene regulation

December 11, 2018

A team of engineers at the University of Delaware has developed a method to use CRISPR/Cas9 technology to set off a cascade of activities in cells, a phenomenon known as conditional gene regulation. Their method, described ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.