Single-molecule localization techniques provide high spatial resolution in protein labeling

Protein labeling with synthetic fluorescent probes is a key technology in chemical biology and biomedical research. The target proximity achieved by small-molecule probes is essential to exploit the full potential of super-resolution fluorescence microscopy.

Single-molecule techniques provide by reporting on the position of the fluorophore and thus only indirectly on the target molecule itself. Large labels, such as antibodies, can misleadingly position a fluorophore tens of nanometers away from the target. Since single-molecule localization can achieve almost the ultimate spatial precision.

To overcome these limitations while at the same time achieving a labeling specificity comparable to that of antibodies, a team of Frankfurt scientists has developed the small labeling pair (SLAP) technology, which fulfills all necessary requirements for single-molecule localization microscopy.

This highly sensitive and efficient modular approach, published in the latest issue of the journal Angewandte Chemie, is based on a synthetic small-molecule recognition unit and the genetically encoded minimal protein His6-10-tag. It avoids masking by large probes and supplies sensitive, precise, and robust size analysis of protein clusters. The efficient and modular technique will pave the way to high-throughput high-resolution localization analysis of almost the entire His-tagged proteome.

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Major innovation in molecular imaging delivers spatial and spectral info simultaneously

More information: "SLAP: Small Labeling Pair for Single-Molecule Super-Resolution Imaging." Angew Chem Int Ed Engl. 2015 Aug 24;54(35):10216-9. DOI: 10.1002/anie.201503215
Journal information: Angewandte Chemie

Citation: Single-molecule localization techniques provide high spatial resolution in protein labeling (2015, September 10) retrieved 21 January 2021 from
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