'Stressed' bacteria become resistant to antibiotics

Bacteria become resistant to antibiotics when stressed, finds research published in BioMed Central's open access journal BMC Evolutionary Biology. In particular E. coli grown at high temperatures become resistant to rifampicin.

It is generally thought that antibiotic resistance is costly to maintain, for example mutations which reduce antibiotic uptake also restrict the amount of nutrients entering the cell. Consequently in the absence of antibiotics non- will out-compete the resistant ones. However researchers from UC Irvine and Faculté de Médicine Denis Diderot have discovered that by putting bacteria under stress, by growing them at a high temperature, the bacteria could spontaneously develop resistance to the antibiotic rifampicin.

The mutations responsible for rifampicin resistance had different effects in other strains of E. coli. In each type of bacteria tested the mutated subunit of the rpoB allowed them to grow in the presence of rifampicin, but unlike the original test strain they did not necessarily have a growth advantage at high temperature.

Dr Olivier Tenaillon who led this study commented, "Our study shows that antibiotic resistance can occur even in the absence of antibiotics and that, depending on the type of bacteria, and growth conditions, rather than being costly to maintain can be highly beneficial. Given that rifampicin is used to treat serious bacterial infections such as tuberculosis, , Legionnaire's disease, and for prophylaxis in cases of meningococcal meningitis, this development has important implications for public health."

These bacteria provide strong evidence that the evolution of antibiotic resistance is governed by two properties of genes, pleiotropy and epistasis. Dr Arjan de Visser from Wageningen University explained, "Pleiotropy describes how the antibiotic resistance mutations affect other functions, hence their fate in other environments. Epistasis describes how well different mutations combine in their effect on resistance, and therefore determines which mutational pathway will be preferred by evolution when several mutations are needed for full resistance."

Explore further

Development of antibiotic resistance more predictable than expected

More information: Evolution of Escherichia coli rifampicin resistance in an antibiotic-free environment during thermal stress, Alejandra Rodríguez-Verdugo, Brandon S Gaut and Olivier Tenaillon, BMC Evolutionary Biology (in press)
Journal information: BMC Evolutionary Biology

Provided by BioMed Central
Citation: 'Stressed' bacteria become resistant to antibiotics (2013, February 21) retrieved 17 August 2019 from https://phys.org/news/2013-02-stressed-bacteria-resistant-antibiotics.html
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Feb 21, 2013
John Cairns, at Harvard, did some wonderful and still hotly debated work on mutations resulting from stress. This led to the theory of directed mutagenesis. It seems as though this could be related. Though I'm less familiar with critiques of Cairns' work, I believe pleiotropy and epistasis was the most likely explanation to what Cairns witnessed in E. coli (probably more likely than directed mutagenesis itself). Cool stuff. Can't wait to hear more.

Feb 22, 2013
*directed mutagenesis HYPOTHESIS

Anyone want proof of evolution? With the disclaimer; "don't try this at home" take massive doses of antibiotics, or not finish a regimen you need, and you to can have proof, in your bowels…oh joy. We scientists need to better explain the liberal use of the word "theory" If something is observed, like apparent graduated change among species; where an embryo of a more "advanced" animal may look a lot like a actual "flesh and blood" animal,the theory part is knowing the mechanism, the "how"

What Darwin (I find it odd,this long dead man, still threatens some peoples world view) did not know is that sometimes rapid change can happen in a generation, called "punctuated equilibrium" But change happens, the why,environmental stress, usually not rapidly, or mutation, is not up for a vote. We also have a lot of "theories" on the mechanism of gravity. You don't have to believe in gravity or evolution. it's OK because it believes in you…

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