Golden Nanotubes Used for Imaging Agent to Detect Tumor Cells, Map Sentinel Lymph Node

Aug 26, 2009
(a) Researchers coated carbon nanotubes with gold and applied a cell-targeting bio-agent to deliver to the target. (b) They targeted endothelial LYVE-1 receptors with antibody golden nanotubes to produce photoacoustic and photothermal signals.

(PhysOrg.com) -- Biomedical researchers at the University of Arkansas and University of Arkansas for Medical Sciences in Little Rock have developed a special contrast-imaging agent that is capable of molecular mapping of lymphatic endothelial cells and detecting cancer metastasis in sentinel lymph nodes. The new material could be used as a more efficient and less toxic alternative to nanoparticles and fluorescent labels used in the non-invasive, targeted molecular detection of normal cells, such as immune-related cells, and abnormal cells, such as cancer cells and bacteria. Findings were published in Nature Nanotechnology.

Research teams led by Jin-Woo Kim, associate professor in the department of biological and agricultural engineering at the University of Arkansas, and Vladimir P. Zharov, professor in the Winthrop P. Rockefeller Cancer Institute at UAMS, worked with carbon nanotubes and gold. In a previous study, Kim and Zharov demonstrated that carbon nanotubes held great promise as contrast agents for photoacoustic detection and photothermal killing of individual bacteria in .

Developed by Zharov, photoacoustic and photothermal methods deliver energy, via laser pulses, into biological tissue. When some of the energy is absorbed and converted into heat, it expands and emits sound waves. However, the carbon nanotubes had not been fully developed as an imaging agent because of concerns about toxicity.

Kim’s research team addressed this problem by depositing a thin layer of gold around the carbon nanotubes. The gold layer enhanced absorption of laser radiation and reduced toxicity. In vitro tests showed only minimal toxicity associated with the golden nanotubes. Compared to existing nanoparticles, the golden nanotubes also exhibited high absorption at a miniscule diameter. The golden nanotubes required extremely low laser-energy levels for detection, and low concentrations were required for effective diagnostic and therapeutic applications.

The synthesis process is simple, inexpensive and environmentally friendly, Kim said. The reaction of the carbon nanotubes and gold chloride occurs in water and happens at ambient temperature. No other chemicals or special conditions, such as heating, are required.

In the recent study, the golden nanotubes were used as contrast agents with the advanced imaging methods. The researchers applied the golden nanotubes to solve the challenging biomedical problem of detecting cancer in the lymphatic system, which is responsible for the process of metastasis.

Specifically, the researchers coupled the golden nanotubes with an antibody specific to a critical lymphatic-endothelial receptor. This enabled the mapping of lymphatic , which line the internal surface of lymphatic vessels. This is important because lymphatic endothelial cells come into direct contact with other cells, such as immune-related cells, tumor cells and bacteria entering the lymphatic system. The specific receptor, known as LYVE-1, is one of the most widely used markers of lymphatic endothelium.

Zharov, Ekaterina Galanzha, research assistant professor at UAMS, and other colleagues on Zharov’s team successfully demonstrated the unique ability of the golden nanotubes for integrated diagnosis and therapy at the single cell level. This included targeted lymphatic mapping and the eradiation of cancer micro-metastasis in the critical sentinel lymph nodes, the first lymph node or group of nodes reached by metastasizing from a primary tumor.

The large surface area on the golden nanotubes may allow bioconjugation, or cooperation, with several biomarkers targeted simultaneously. Absorption can be adjusted for multi-color targeting because the size of the golden nanotubes can be changed easily by modulating the length and diameter of nanotube core and thickness of the gold layer, Kim said. This advantage may increase the percentage of detectable cells within a targeted cell population and may allow multiple targets to be detected simultaneously. Also, the golden nanotubes’ hollow core could carry therapeutic agents, such as drugs, magnetic materials, ethanol and other chemicals, to further enhance their therapeutic effects.

Provided by University of Arkansas (news : web)

Explore further: Innovative strategy to facilitate organ repair

add to favorites email to friend print save as pdf

Related Stories

Scientists fight cancer with nanotechnology

May 21, 2009

(PhysOrg.com) -- Nanotechnology researchers at the University of Arkansas at Little Rock have developed a method of detecting, tracking, and killing cancer cells in real time with carbon nanotubes.

Polymer Nanotubes as Molecular Probes and DNA Carriers

May 01, 2006

By growing polymers on a porous aluminum oxide template, researchers at the Seoul National University in Korea have fabricated polymer nanotubes to which they can attach two different types of molecules. These new nanoscale ...

Carbon nanotube absorption measured in worms, cancer cells

Mar 28, 2006

University of Michigan researchers have discovered how to measure the absorption of multi-walled carbon nanoparticles into worms and cancer cells, a breakthrough that will revolutionize scientists' understanding of how the ...

Recommended for you

Innovative strategy to facilitate organ repair

Apr 18, 2014

A significant breakthrough could revolutionize surgical practice and regenerative medicine. A team led by Ludwik Leibler from the Laboratoire Matière Molle et Chimie (CNRS/ESPCI Paris Tech) and Didier Letourneur ...

Physicists create new nanoparticle for cancer therapy

Apr 16, 2014

A University of Texas at Arlington physicist working to create a luminescent nanoparticle to use in security-related radiation detection may have instead happened upon an advance in photodynamic cancer therapy.

User comments : 0

More news stories

NASA's space station Robonaut finally getting legs

Robonaut, the first out-of-this-world humanoid, is finally getting its space legs. For three years, Robonaut has had to manage from the waist up. This new pair of legs means the experimental robot—now stuck ...

Ex-Apple chief plans mobile phone for India

Former Apple chief executive John Sculley, whose marketing skills helped bring the personal computer to desktops worldwide, says he plans to launch a mobile phone in India to exploit its still largely untapped ...

Filipino tests negative for Middle East virus

A Filipino nurse who tested positive for the Middle East virus has been found free of infection in a subsequent examination after he returned home, Philippine health officials said Saturday.

Egypt archaeologists find ancient writer's tomb

Egypt's minister of antiquities says a team of Spanish archaeologists has discovered two tombs in the southern part of the country, one of them belonging to a writer and containing a trove of artifacts including reed pens ...