Study shows ACKR1 is not expressed on monocytes and macrophages, despite previous conclusions

antibodies
Credit: Unsplash/CC0 Public Domain

Antibodies are among the most frequently used tools in the biosciences, as they enable researchers to identify molecules. However, many commercial antibodies are not specific enough, with the result that they do not recognize the molecules they are supposed to target.

A team led by Dr. Johan Duchêne (Institute for Cardiovascular Prevention) has now investigated commercial antibodies against the molecule ACKR1, which is expressed on , endothelial cells, and some neurons. On , it is an important regulator of inflammation, as it controls the migration of white blood cells into tissue. It had been shown that the malaria pathogen uses ACKR1 to infect red blood cells.

A study from 2016 reported that ACKR1 is also expressed on monocytes and macrophages. This would have influenced prevailing assumptions about the pathophysiological functions of ACKR1 and could raise the question, for example, as to whether the also infects macrophages and monocytes.

Duchêne has now refuted this study by demonstrating that the do not recognize the molecule and ACKR1 is not expressed by macrophages and monocytes. Reproducibility and replicability of research results are important quality criteria in science. As the authors emphasize, their study exemplifies the importance for the scientific community of publishing corrections.

The research was published in Cell Stem Cell.


Explore further

How ancestry shapes our immune cells

More information: Antal Rot et al, Murine bone marrow macrophages and human monocytes do not express atypical chemokine receptor 1, Cell Stem Cell (2022). DOI: 10.1016/j.stem.2021.11.010
Journal information: Cell Stem Cell

Citation: Study shows ACKR1 is not expressed on monocytes and macrophages, despite previous conclusions (2022, July 8) retrieved 26 September 2022 from https://phys.org/news/2022-07-ackr1-monocytes-macrophages-previous-conclusions.html
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
13 shares

Feedback to editors