Separating tiny bacteria by shape: Simple tech for E. coli sorting

Separating tiny bacteria by shape: Simple tech for E. coli sorting
The design of the viscoelastic microfluidic device for shape-based separation of drug-treated E. coli. Schematics of the microfluidic device used for E. coli separation by shape. Credit: Nara Institute of Science and Technology

Scientists at Nara Institute of Science and Technology (NAIST) achieved shape-based separation of Escherichia coli (E. coli) bacteria using a viscoelastic fluid inside microfluidic channels. This "lab-on-a-chip" system has the capability to assist in making scientific experiments more reproducible, as well as provide more accurate assessments of the severity of bacterial infections based on patient samples.

The task of separating tiny based on their shape is a vital step in scientific and clinical applications, but also a difficult one. Whether an E. Coli bacterium is in a round or elongated configuration can indicate its state of biological function, and the ability to prepare homogeneous populations with uniform shapes may help improve experimental reproducibility. Moreover, being able to sort samples into sub-populations based on their shape may help diagnose patient health or assess environmental contamination. However, this ability has been difficult to achieve, especially at the scales needed to be practical.

Now, a team of researchers at NAIST has been able to separate drug-treated E. coli bacteria using a microfluidic channel containing poly-ethylene-oxide (PEO), a viscoelastic fluid. The elongation of the bacteria, as measured by the aspect ratio, ranged from 1.0 (spherical) to 5.5 (rod-shaped). Microchannels made of poly(dimethylsiloxane) were created with photolithography. PEO, which has both high viscosity and elasticity, was made to flow along the edges of an expanding V-shaped chamber. The elongated bacterium followed this flow to be collected at the edges of the device.

"The shape of a E. coli bacterium changes based on its attachment, motility, dispersal, predation and differentiation behaviors," author Yalikun Yaxiaer explains. A rod-like configuration is more efficient for swimming, while a may help it form communities. These changes are important milestones for the progression of disease, and whether the bacteria are swimming free or forming communities.

"This technology will allow downstream genomics experiments on the separated E. coli populations to determine linkage between cell shape, antibiotic susceptibility and gene function," author Yoichiroh Hosokawa says. The team hopes the microfluidic platform they developed will be adopted by the broader scientific community for different applications in the fields of microbiology, molecular biology and biomedicine. Their research is published in Lab on a Chip.

More information: Tianlong Zhang et al, Shape-based separation of drug-treated Escherichia coli using viscoelastic microfluidics, Lab on a Chip (2022). DOI: 10.1039/D2LC00339B

Journal information: Lab on a Chip

Provided by Nara Institute of Science and Technology

Citation: Separating tiny bacteria by shape: Simple tech for E. coli sorting (2022, June 9) retrieved 2 March 2024 from
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.

Explore further

Examining cell shapes without a microscope


Feedback to editors