Exponential scaling of frictional forces in cells

Exponential scaling of frictional forces in cells
A polymer composed of a protein tubulin, it is a component of the cytoskeleton involved in intracellular transport, cellular mobility and nuclear division. Credit: AMOLF

AMOLF researchers have presented a theory that describes the friction between biological filaments that are crosslinked by proteins. Surprisingly, their theory predicts that the friction force scales highly nonlinearly with the number of crosslinkers. The authors believe that cells use this scaling not only to stabilize cellular structures, but also to control their size. The new findings are important for the understanding of the dynamics of cellular structures such as the mitotic spindle, which pulls chromosomes apart during cell division.

Motor proteins versus frictional forces

Many consist of long filaments that are crosslinked by motor proteins and non-motor proteins (see figure). These so-called cytoskeletal structures not only give cells their mechanical stability, but also enable them to crawl over surfaces and to pull chromosome apart during . Force generation is typically attributed to motor proteins, which, using chemical fuel, can move the filaments with respect to one another. However, these motor forces are opposed by frictional forces that are generated by passive, non-. These frictional forces are a central determinant of the mechanical properties of cytoskeletal structures, and they limit the speed and efficiency with which these structures are formed. Moreover, they can even be vital for their stability, because if the motor forces are not opposed by the forces generated by the passive crosslinkers, the structures can even fall apart.

Exponential increase

To understand the dynamics of these cytoskeletal structures and the forces they can generate, it is essential to understand how the frictional forces scale with the length of the filaments and the number of crosslinkers between them. Existing theories predict that friction increases linearly with the number of crosslinkers, which is what one would expect intuitively. However, recent experiments have vividly demonstrated that the friction forces scale non-linearly, i.e. exponentially, with the number of crosslinkers. Due to the COVID-19 crisis, we all know what a dramatic difference an exponential versus a linear increase can make. Up until now, the origin of this highly unusual exponential scaling behavior of the friction between filaments was not understood.

Exponential scaling of frictional forces in cells
Cytoskeletal structures consist of filaments that are crosslinked by proteins. These filaments consist of a regular lattice of subunits, which yield a discrete set of binding sites for the crosslinkers. The theory of the authors predicts that, as a result, the friction forces scale very rapidly as the number of crosslinkers increases. Credit: AMOLF

Explanation

AMOLF group leader Ten Wolde and Ph.D. student Wierenga have now developed a theory that explains these experimental observations. Their theory is based on the observation that biological filaments consist of a regular lattice of subunits, yielding a discrete set of binding sites for the crosslinkers. Ten Wolde and Wierenga predict that the filaments can only move if the linkers re-organize collectively. As a result of this collective reorganization, the friction forces increase very rapidly, i.e., exponentially, with the number of linkers.

The work of the authors has major implications for our understanding of the dynamics of cytoskeletal structures. In particular, the exponential scaling means that these structures essentially freeze when the crosslinker density exceeds a certain threshold; the frictional forces become so high that they prohibit any further movement. Cells may use this strong scaling to control the size and stability of cellular structures.


Explore further

The rub with friction: Researchers uncover new rules of friction at microscopic level

More information: Harmen Wierenga et al. Diffusible Cross-linkers Cause Superexponential Friction Forces, Physical Review Letters (2020). DOI: 10.1103/PhysRevLett.125.078101
Journal information: Physical Review Letters

Provided by AMOLF
Citation: Exponential scaling of frictional forces in cells (2020, August 14) retrieved 27 September 2020 from https://phys.org/news/2020-08-exponential-scaling-frictional-cells.html
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
78 shares

Feedback to editors

User comments