Scientists find precise control of terminal division during plant stomatal development

Scientists find precise control of terminal division during plant stomatal development
The model for RPA2 function in stomatal terminal division regulation and DNA repair progression Credit: IBCAS

Stomata are plant-specific epidermal structures that consist of paired guard cells surrounding a pore. The opening and closing of these micro-valves facilitate carbon dioxide uptake for photosynthesis and reduce excessive water loss in plants.

Recently, a research group led by Prof. Le Jie at the Institute of Botany of the Chinese Academy of Sciences (IBCAS) found a genetic suppressor of flp stomatal defects. They found that RPA2a, a core subunit of Replication Protein A (RPA) complexes, acted downstream from the core cell cycle genes of CDKB1 to ensure terminal division during stomatal development and the formation of paired to create functional units.

RPA is a heterotrimeric single-stranded DNA (ssDNA)-binding that is required for multiple aspects of DNA metabolism, including DNA replication, recombination, and repair. The homologues of each of the three RPA subunits (RPA1-3) are well conserved in eukaryotes, including humans.

Le's group demonstrated that CDK-mediated phosphorylation at the N-terminus of RPA2a was essential for RPA functioning and localization. The scientists also showed that Serine-11 and Serine-21 are evolutionarily conserved CDK-phosphorylation sites. Furthermore, their results revealed that being phosphorylated by CDK was required for RPA2a to respond to DNA damage.

The study, entitled "A conserved but plant specific CDK-mediated regulation of DNA replication protein A2 in the precise control of stomatal terminal division," was published online in PNAS on August 20, 2019. Yang Kezhen is the first author and Le Jie is the corresponding author.


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More information: Kezhen Yang et al, A conserved but plant-specific CDK-mediated regulation of DNA replication protein A2 in the precise control of stomatal terminal division, Proceedings of the National Academy of Sciences (2019). DOI: 10.1073/pnas.1819345116
Citation: Scientists find precise control of terminal division during plant stomatal development (2019, August 21) retrieved 21 September 2019 from https://phys.org/news/2019-08-scientists-precise-terminal-division-stomatal.html
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