Building a flu factory from host cell components

Building a flu factory from host cell components
A quantitative proteomic study of how influenza virus affects lung cell lines found that in infected cells, the protein synthesis machinery moves from its usual location into the autophagosome, where new flu particles (like the one shown in this picture) are built. Credit: National Institute of Allergy and Infectious Diseases, National Institutes of Health

Perhaps inspired by the annual 3 to 5 million cases of severe influenza worldwide, the Guinness World Record organization is advertising for individuals or organizations to attempt a record for the most people getting a flu awareness lesson at once. Meanwhile, a smaller group of people is making a more focused attempt to learn about lots of flu proteins.

Andrea C. Becker at the University of Freiburg and her colleagues in Germany and Switzerland investigated the effect of the flu-causing virus, influenza A, on three lung-derived cell lines and published their results in the journal Molecular & Cellular Proteomics.

The study quantified virus-induced changes in levels and found that a majority of the changes are cell-line specific. More specifically, they quantified the of 70 percent of the roughly 7,000 proteins they could detect using a mass spectrometry-based isotope labeling approach known as SILAC.

Influenza infection of lung cells changed the overall abundance of only a few proteins, mostly related to immunity, but SILAC comparisons showed that the virus changes the cellular location of many proteins. In particular, the authors detected an increase in viral and ribosomal proteins in the autophagosome, which they linked to a reduction in successful autophagy, suggesting that the virus may hijack autophagosomes, perhaps using the compartments for viral protein translation.

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More information: Andrea C Becker et al, Influenza A virus induces autophagosomal targeting of ribosomal proteins, Molecular & Cellular Proteomics (2018). DOI: 10.1074/mcp.RA117.000364
Journal information: Molecular & Cellular Proteomics

Citation: Building a flu factory from host cell components (2018, September 28) retrieved 26 October 2020 from
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