New testing finds synergistic combination leads to toxicity in nanomaterials

June 29, 2018, University of Oregon
New testing finds synergistic combination leads to toxicity in nanomaterials
Credit: ACS

A new study finds reason for caution—a clear emergence of toxicity—in nanomaterial product formulations, but it also provides an early testing technique that could help the industry continue to move forward.

In a surprise discovery, University of Oregon chemists and Oregon State University toxicologists found that biocompatible gold nanoparticles and widely used surfactants—each previously considered safe as individual components—become toxic to zebrafish embryos as they combine in a synergistic way.

The synergistic, or multiplicative effect, was discovered when using a new delivery system while testing nanomaterials, the four-member research team noted in a paper placed online April 26 and in print June 26 in ACS Nano.

"Years after showing that these materials were the most benign and among the least toxic materials that we've ever seen, we did these experiments with the surfactants and found that, in this case, they were toxic," said co-author Jim Hutchison of the UO's Department of Chemistry and Biochemistry.

"Our new study gives us a wakeup call," he said. "This isn't the first time that people have seen mixture toxicity, but it does remind us that two safe things mixed together doesn't mean that the mixture is safe."

It is not clear, he said, if toxicity in zebrafish suggests a threat to human health.

In nanotechnology's infancy, toxicologists hand delivered nanoparticles to zebrafish using pipettes. Hutchison and OSU co-author Robert Tanguay had previously had found that inorganic nanoparticles and surfactants, individually, were not toxic to zebrafish.

However, a switch to automation—using inkjet-printer-like devices to rapidly inject materials employing small amounts of surfactant to control the size of the delivered droplets—brought unforeseen effects.

The new study found an 88 percent mortality rate of zebrafish embryos exposed to gold nanoparticles mixed with polysorbate 20, whereas pipette delivery of the nanoparticles alone resulted in just 3 percent toxicity.

Polysorbates are surfactants and emulsifiers commonly used in laundry detergent, suntan lotions, cosmetics and ice cream. The team also found synergistic toxicity using two other common surfactants, polysorbate 80 and sodium dodecyl sulfate.

In the project, the researchers re-examined the toxicity of nanoparticles that had been studied as part of the Safer Nanomaterials and Nanomanufacturing Initiative, which was funded by the Air Force Research Laboratory. The National Science Foundation and National Institutes of Health supported the recent work.

The cause of the synergistic toxicity was discovered when materials were analyzed with diffusion-ordered NMR spectroscopy, an adaptation of nuclear magnetic resonance that reveals how particles move, or diffuse, in solution.

As increasing amounts of surfactants were added, the researchers observed that the particles diffused more slowly because the assembles on the outside of the gold nanoparticles, leading to both increased uptake and toxicity, driven by surface structure, in the zebrafish.

"Our NMR method allowed us to state that this synergistic toxicity is really related to the interaction of these two benign materials and that it generates something that is more toxic," Hutchison said.

The rapid-screening approach used in the research, he said, could serve as an early screening method. It would allow for adjustments in formulations or the redesign of individual ingredients before large investments have been made, to assure that products are safe, Hutchison said.

"Zebrafish have proven to be a powerful high-throughput screening laboratory model that helps us to rapidly discover if chemical mixtures are hazardous," Tanguay said. "It's not a human, but the biological similarities between human and zebrafish are remarkably high, so findings in zebrafish often predict human hazards."

Hutchison and Tanguay are internationally known for pioneering the use of green chemistry, also known as sustainable chemistry, in designing . The technique harnesses molecular design principles to produce safer chemicals, reduce and minimize waste.

Aurora L. Ginzburg, a UO doctoral student working on the synthesis of for biomedical uses, led the analysis efforts using equipment in the UO's Center for Advanced Materials Characterization in Oregon, known as CAMCOR. Lisa Truong, deputy director of OSU's Sinnhuber Aquatic Research Laboratory, also was a co-author. Her focus is utilizing the developmental model to assess the safety of chemicals and products in commerce.

Explore further: Green chemistry can help nanotechnology mature, professor says

More information: Aurora L. Ginzburg et al, Synergistic Toxicity Produced by Mixtures of Biocompatible Gold Nanoparticles and Widely Used Surfactants, ACS Nano (2018). DOI: 10.1021/acsnano.8b00036

Related Stories

Green chemistry can help nanotechnology mature, professor says

February 19, 2007

“Around the world, there is a growing urgency about nanotechnology and its possible health and environmental impacts,” Hutchison said in his talk Sunday during a workshop at the annual meeting of the American Association ...

The impact of anti-odor clothing on the environment

March 30, 2016

Anti-odor athletic clothes containing silver nanoparticles have gained a foothold among exercise buffs, but questions have arisen over how safe and effective they are. Now scientists report in ACS' journal Environmental Science ...

Lungs may suffer when certain elements go nano

January 28, 2014

(Phys.org) —Nanoparticles are used in all kinds of applications—electronics, medicine, cosmetics, even environmental clean-ups. More than 2,800 commercially available applications are now based on nanoparticles, and by ...

Recommended for you

Single-celled architects inspire new nanotechnology

July 16, 2018

Diatoms are tiny, unicellular creatures, inhabiting oceans, lakes, rivers, and soils. Through their respiration, they produce close to a quarter of the oxygen on earth, nearly as much as the world's tropical forests. In addition ...

X-ray triggered nano-bubbles to target cancer

July 16, 2018

Innovative drug filled nano-bubbles, able to be successfully triggered in the body by X-rays, have been developed by researchers, paving the way for a new range of cancer treatments for patients.

Smart window controls light and heat, kills microorganisms

July 13, 2018

A new smart window offers more than just a nice view—it also controls the transmittance of sunlight, heats the interiors of buildings by converting solar radiation into heat, and virtually eliminates E. coli bacteria living ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.