New technology makes oak wilt detection faster, more affordable

New technology makes oak wilt detection faster, more affordable
The microfluidic device used to isolate the oak wilt fungi from wood chip samples. Credit: University of Minnesota

Oak wilt fungus is another invasive plant pathogen that often goes unnoticed, until it's too late. Not anymore. New technology developed by University of Minnesota College of Food, Agricultural and Natural Resource Sciences (CFANS) Assistant Professor Abdennour Abbas and his research team offers a simple, affordable diagnostic test utilizing nanotechnology and gold.

"Oak wilt is the second greatest invasive disease threat to Minnesota's plants, second only to Dutch elm disease. The loss of elms transformed many of our urban and hardwood forests. We don't want our oaks to be next," said Robert Venette, Director of the Minnesota Invasive Terrestrial Plants and Pests Center (MITPPC) at the University of Minnesota.

The spread of oak wilt is sobering and serious. According to the USDA Forest Service, oak wilt fungus is present in 21 eastern states. More than 266,000 were infected by oak wilt fungus between 2007 and 2016 in Minnesota. The fungus is found spanning 25 counties including Sherburne, Anoka, Isanti and northwestern Dakota counties.

Currently, oak wilt detection is performed by visual diagnostic (naked eye observation), which is only possible two to three weeks after infection, or with laboratory techniques that take from six hours to two weeks and cost $60 to $120 per sample. In the field, the symptoms of oak wilt can be confused with drought stress or insect damage.

The cost of infected tree removal alone is estimated to be up to $60 million at around $400 to $500 per tree. That doesn't include the impact on related economic activities. Oak represent not only an important natural habitat for wildlife, but also are a natural resource for firewood, furniture, construction and livestock feed.

The innovation by Abbas' research lab enables oak wilt detection within 30 minutes of sampling and at a fraction of the cost: less than five dollars per sample. The technology uses the agglomeration of gold nanoparticles to generate a chemiluminescent signal that can be read by a hand-held reader, in the presence of the oak wilt fungus DNA. The team is now refining a portable system that allows early detection of the disease in the field without sending samples to the laboratory. The requires only dipping wood chips obtained from infected trees into a first solution to extract the DNA, then mixed with a second solution of to allow detection using a hand-held luminometer.

"When it comes to disease detection, time is a crucial factor that decides whether lives will be saved or not. However, making a rapid detection technology is not enough. The other challenge is to make the affordable and easy for people to use it," said Abbas.

"This is exactly the kind of innovation that we knew would be possible when our Center was created. MITPPC brings new talent and new solutions to the threats posed by terrestrial invasive species," said Venette.

Explore further

Plant disease clinic identifies new case of oak wilt

More information: Renu Singh et al. Rapid and PCR-free DNA Detection by Nanoaggregation-Enhanced Chemiluminescence, Scientific Reports (2017). DOI: 10.1038/s41598-017-14580-w

Renu Singh et al. Microbial separation from a complex matrix by a hand-held microfluidic device, Chem. Commun. (2017). DOI: 10.1039/C7CC06310E

Journal information: Scientific Reports

Citation: New technology makes oak wilt detection faster, more affordable (2017, November 9) retrieved 4 October 2022 from
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