Novel nano-CT device creates high-resolution 3-D X-rays of tiny velvet worm legs

Computer tomography (CT) is a standard procedure in hospitals, but so far, the technology has not been suitable for imaging extremely small objects. In PNAS, a team from the Technical University of Munich (TUM) describes a nano-CT device that creates three-dimensional X-ray images at resolutions up to 100 nanometers. Together with colleagues from the University of Kassel and Helmholtz-Zentrum Geesthacht, the researchers have analyzed the locomotory system of a velvet worm.

During a CT analysis, the object under investigation is X-rayed and a detector measures the respective amount of radiation absorbed from various angles. Three-dimensional images of the inside of the object are constructed based on several such measurements. However, the technology reached its limits when it came to objects as small as the tiny, 0.4 millimeter long legs of the velvet worm (Onychophora).

High-resolution images of this magnitude require radiation from particle accelerators, yet there are only a few dozen such facilities in Europe. Approaches suitable for the typical laboratory still had to struggle with low resolutions, and were limited to certain materials and could not exceed a certain size. The reason was often the use of X-ray optic, which focus X-ray radiation in a way similar to optical lenses focusing light—but they also have several limitations.

The TUM Nano-CT system is based on a newly developed X-ray source that generates a particularly focused beam without relying on X-ray optics. In combination with an extremely low-noise detector, the device produces images that approach the resolution of a scanning electron microscope, while also capturing structures under the surface of the target. "Our system has decisive advantages compared to CTs using X-ray optics," says TUM scientist Mark Müller, lead author of the PNAS article. "We can make tomographies of significantly larger samples and we are more flexible in terms of the materials that can be investigated."

These properties were ideal for the team led by Prof. Georg Mayer, head of the Department of Zoology at the University of Kassel. The scientists investigate the evolutionary origin of arthropods, including, for example, insects, spiders and crustaceans. Their current research, however, focuses on velvet worms (onychophorans), which can be thought of as worms with legs. They are closely related to arthropods. Some velvet worm species can grow to a length of up to 20 centimeters, while others do not exceed one centimeter. The exact zoological classification of these ancient animals is still a matter of controversy; presumably, they share a common ancestor with arthropods and tardigrades (water bears).

"In contrast to arthropods, onychophorans do not have segmented limbs, as is also the case with their presumed common fossil ancestors," says Georg Mayer. "The investigation of the functional anatomy of the velvet worm's legs plays a key role in determining how the segmented limbs of the arthropods evolved." The Nano-CT images make it possible to investigate the individual muscle strands of the velvet worm leg. The team from Kassel plans to publish detailed results in the next few months. But they are already convinced about one thing: The Nano-CT device has passed its first practical test.

"In the future, this technology will also make biomedical investigations possible. Thus, for example, we will be able to examine tissue samples to clarify whether or not a tumor is malignant. A non-destructive and three-dimensional image of the tissue with a resolution like that of the Nano-CT can also provide new insights into the microscopic development of widespread illnesses such as cancer."